Anti-CD105/Endoglin Rabbit Monoclonal Antibody for WB, FC, ELISA - Q63961

Core Product Specifications and Parameters

Biological Background: CD105/Endoglin Function and Localization

• Endoglin is a vascular endothelium glycoprotein crucial for angiogenesis, required for normal extraembryonic angiogenesis and embryonic heart development. Related references: PMID:10625534
• It regulates vascular endothelial cell migration, contributing to vascular remodeling and maintenance of adult vasculature integrity. Related references: PMID:17540773
• Acts as a TGF-beta coreceptor, involved in the TGF-beta/BMP signaling cascade leading to SMAD transcription factor activation, modulating both TGFB1 and GDF2/BMP9 pathways. Related references: PMID:23300529
• May regulate endothelial cell shape changes in response to blood flow, driving vascular remodeling and normal morphology during angiogenesis. Related references: PMID:28530658
• Subcellular localization: Cell membrane; contains a signal peptide and a single-pass transmembrane domain.
• Tissue specificity: Detected on blood vessels, including pulmonary artery and cardiac capillaries; also found in stromal cells of various tissues. Related references: PMID:8194490, PMID:10625534
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation (based on UniProt keywords).

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 70 kDa is expected under reducing conditions; validate with appropriate positive controls such as endothelial cell lysates.
• For flow cytometry (FC), the antibody targets the extracellular domain (aa 27–581), making it suitable for live cell surface staining without permeabilization.
• For ELISA, the recombinant immunogen can serve as a positive control; consider pairing with a detection antibody for sandwich ELISA development.
• Validate antibody performance in your specific sample system and application, as experimental conditions may influence results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive supply of IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk reagents. For CD105/Endoglin and other angiogenesis-related targets, we support sourcing and procurement to help streamline your assay development from concept to clinic. Our one-stop services cover technical consultation and reliable material supply to meet your research and diagnostic needs.