Anti-CDK5R1 Monoclonal Antibody for WB, ELISA - Q15078

Core Product Specifications and Parameters

Biological Background: CDK5R1 Function and Localization

• CDK5R1 encodes p35, a neuron-specific activator of cyclin-dependent kinase 5 (CDK5), and is also known as p25 or p23 due to proteolytic processing.
• The p35/CDK5 complex is essential for neurite outgrowth and cortical lamination during brain development.
• It participates in dendritic spine morphogenesis by mediating EFNA1-EPHA4 signaling.
• p35/CDK5 regulates the circadian clock by phosphorylating CLOCK at Thr-451 and Thr-461, modulating CLOCK-BMAL1 transcriptional activity and subcellular distribution.
• Subcellularly, CDK5R1 localizes to the cell membrane, neuron projections, cytoplasm, perinuclear region, and nucleus.
• Expression is highly restricted to the brain and neurons.
• Key post-translational modifications include phosphorylation and myristoylation, and the protein is involved in biological rhythms.

Experimental Guidance and Technical Tips

• The immunogen (aa 200–300) may detect both full-length p35 and the truncated p25 fragment if the epitope is conserved; validate in the relevant sample system.
• Recommended applications are Western blotting (WB) and ELISA; consider optimizing antibody dilutions using brain tissue lysates or recombinant protein.
• For WB, a band around 34 kDa is expected; additional bands may correspond to cleavage products or isoforms.
• Given its neuron specificity, ensure sample preparation includes appropriate brain or neuronal cell extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as your comprehensive IVD raw-material sourcing partner, from concept to clinic. For neuroscience targets like CDK5R1, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Contact us for competitive procurement of rare target raw materials.