Anti-CEACAM5 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• Cell surface glycoprotein playing a role in cell adhesion, intracellular signaling, and tumor progression. Related references: PMID:10864933, PMID:10910050, PMID:2803308
• Mediates homophilic and heterophilic cell adhesion with other CEACAM family members, such as CEACAM6. Related references: PMID:2803308
• Functions as an oncogene by inducing resistance to anoikis in colorectal carcinoma cells. Related references: PMID:10910050
• Acts as a receptor for E.coli Dr adhesins, with binding leading to homodimer dissociation.
• Localized primarily to the cell membrane, with enrichment on apical surfaces.
• Expressed in columnar epithelial and goblet cells of the colon, and found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Related references: PMID:10436421
• Glycoprotein with a GPI-anchor membrane attachment; contains multiple immunoglobulin-like domains and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 35‑685), which includes multiple Ig‑like domains and the GPI‑anchor signal sequence, potentially providing broad epitope coverage for detecting both full‑length and soluble forms of CEACAM5.
• For Western blotting, the predicted molecular weight is 77 kDa; however, extensive glycosylation may result in a higher apparent molecular weight. Validate lysate preparation and antibody dilution in the relevant sample system.
• For immunohistochemistry (paraffin), standard antigen retrieval methods are recommended. Optimization of blocking and detection conditions may be necessary due to the glycoprotein nature and tissue‑specific expression of CEACAM5.
• In ELISA, consider using a paired detection antibody recognizing a distinct epitope to enhance assay specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, clinical laboratories, and research institutes with streamlined access to raw materials for CEACAM5‑related immunoassay development, including optimized monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Our one‑stop sourcing platform bridges the gap from early‑stage target validation to commercial kit production, particularly for cancer biomarker and cell adhesion applications. Explore our portfolio for CEACAM5 and other carcinoembryonic antigen family members to accelerate your diagnostic assay pipeline.