Anti-CCL5 Polyclonal Antibody for WB, ELISA - P50231

Core Product Specifications and Parameters

Biological Background: CCL5 Function and Localization

• CCL5, also known as RANTES, SIS-delta, and Small-inducible cytokine A5, is a member of the C-C chemokine family.
• It acts as a chemoattractant for blood monocytes, memory T-helper cells, and eosinophils, and it causes histamine release from basophils and activates eosinophils.
• CCL5 may activate several chemokine receptors including CCR1, CCR3, CCR4, and CCR5, and it may serve as an agonist for the G protein-coupled receptor GPR75, contributing to neuron survival and insulin secretion.
• The protein is secreted and contains a signal peptide and disulfide bonds, characteristic of chemokines involved in inflammatory responses.
• The rat CCL5 gene (Ccl5/Scya5, GeneID 81780) shares homology with the human ortholog (GeneID 6352).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 29-52 of rat CCL5 (AAA96499.1). For Western blot (WB), a band around 10 kDa is expected for the mature protein, but consider potential glycosylation or dimerization that may affect migration.
• For ELISA applications, the antibody may be used to detect rat CCL5 in cell supernatants or serum; validate the assay conditions in the relevant sample matrix.
• Since CCL5 is secreted, it is important to assess its expression levels in the appropriate biological context (e.g., stimulated immune cells) for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. We specialize in chemokine and inflammation-related targets like CCL5, providing reliable sourcing solutions for research and kit manufacturing.