Anti-CLEC5A Rabbit Polyclonal Antibody for WB and ELISA - Q9NY25

Core Product Specifications and Parameters

Biological Background: CLEC5A Function and Localization

• CLEC5A, also known as myeloid DAP12-associating lectin 1 (MDL-1) or CLECSF5, is a type II transmembrane protein belonging to the C-type lectin domain family.

• Functions as a cell surface receptor that signals via TYROBP, acting as a positive regulator of osteoclastogenesis and inflammatory responses.
  Related references: PMID:10449773

• Serves as a critical macrophage receptor for dengue virus serotypes 1-4. Binding of the virus triggers TYROBP phosphorylation and pro-inflammatory cytokine release, but does not mediate viral entry.
  Related references: PMID:18496526 PMID:21566123

• Localized to the cell membrane.

• Highly expressed in bone marrow, with lower levels in synovium, lung, and bronchus. Expressed in peripheral blood monocytes and monocyte/macrophage cell lines, but down-regulated upon monocyte differentiation into dendritic cells.
  Related references: PMID:20212065 PMID:10449773

• Contains a single transmembrane domain and a C-type lectin domain; classified as a host cell receptor for virus entry and involved in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 29-188) of human CLEC5A, suggesting recognition of the native protein and applicability for detecting both endogenous and recombinant CLEC5A.

• For Western blot, optimize blocking and antibody dilution; the predicted molecular weight of CLEC5A is approximately 22 kDa, so use appropriate gel acrylamide percentage and transfer settings for low-molecular-weight proteins.

• The antibody is reported to cross-react with human, mouse, and rat CLEC5A, making it suitable for cross-species studies; however, validate specificity in your specific model system using appropriate controls.

• For ELISA, this polyclonal antibody may be used as a capture or detection reagent; consider pairing with a monoclonal antibody for enhanced specificity, and optimize assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our CLEC5A polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for research on innate immunity and infectious diseases. Contact us to discuss your raw-material sourcing needs, including rare targets and custom development.