Anti-CCL2/MCP-1 Polyclonal Antibody for WB and ELISA - P13500

Core Product Specifications and Parameters

Biological Background: CCL2/MCP-1 Function and Localization

• CCL2/MCP-1 binds to and signals through CCR2, triggering strong chemotaxis and intracellular calcium mobilization. Related references: PMID:10529171, PMID:10587439, PMID:9837883
• It exhibits selective chemotactic activity for monocytes and basophils, but not neutrophils or eosinophils. Related references: PMID:8195247, PMID:8627182, PMID:9792674
• CCL2 may play a role in the recruitment of monocytes into the arterial wall during atherosclerosis. Related references: PMID:8107690
• It is a secreted chemokine, also known as MCP-1, MCAF, HC11, and SCYA2.
• The mature protein can undergo glycosylation and contains conserved disulfide bonds essential for structure.
• Expressed at the protein level in seminal plasma, endometrial fluid, follicular fluid, and monocytes. Related references: PMID:23765988, PMID:2513477

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature peptide (aa 22–99), lacking the signal sequence; this region is conserved in human CCL2.
• Predicted molecular weight of mature CCL2 is ~11 kDa; glycosylation and dimerization may yield higher bands in WB. Consider reducing conditions and validate with appropriate controls.
• For ELISA, pair with a matched detection antibody recognizing a distinct epitope. As a polyclonal antibody, verify specificity against relevant matrix components.
• Due to the secretory nature of CCL2, test conditioned media, serum, or plasma; include negative controls to confirm signal specificity.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one‑stop access to validated IVD raw materials, including optimized polyclonal antibodies like this anti‑CCL2/MCP‑1 antibody for chemokine research and immunoassay development. We offer tailored sourcing of antibody pairs, bulk ancillary reagents, and rare‑target raw materials to accelerate your project from concept to clinic.