Anti-CCR5 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P51681

Core Product Specifications and Parameters

Biological Background: CCR5 Function and Localization

• CCR5, also known as CD195, is a C-C chemokine receptor type 5, a member of the G protein-coupled receptor (GPCR) superfamily.
• It functions as a receptor for inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta, and RANTES, transducing signals by increasing intracellular calcium levels and participating in T-lymphocyte migration to infection sites. Related references: PMID:30713770
• Additionally, CCR5 acts as a coreceptor for HIV-1 viral entry, with CD4 serving as the primary receptor.
• Subcellularly, CCR5 is localized to the cell membrane.
• The protein is highly expressed in spleen, thymus, myeloid and promyeloblastic cell lines, and on CD4+ and CD8+ T-cells, with medium expression in peripheral blood leukocytes and small intestine, and low in ovary and lung.
• Key post-translational modifications and structural features include disulfide bonds, glycosylation, palmitoylation, phosphorylation, and sulfation, which are critical for its function and stability.
• CCR5 is implicated in diabetes mellitus and host-virus interactions, highlighting its role in both physiological and pathological processes.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a synthetic peptide from the C-terminal region (amino acids 246-345) of human CCR5; consider this immunogen location when interpreting target specificity.
• For Western blot (WB), expect a specific band around 41 kDa; use appropriate positive and negative controls to validate signal specificity.
• In immunofluorescence (IF/ICC, IF-P), test antibody performance in CCR5-expressing cells like THP-1 or peripheral blood mononuclear cells (PBMCs) and optimize fixation/permeabilization conditions.
• For ELISA, determine optimal coating antigen concentration and antibody dilution through checkerboard titration; consider using recombinant CCR5 or peptide as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, offering comprehensive access to validated antibodies, bulk reagents, and rare target sourcing. This CCR5 antibody supports research in chemokine signaling, immunology, and HIV infection—areas where CamelBio’s supply chain expertise ensures you receive high-quality materials for diagnostic development. From feasibility to scale-up, we provide the technical and logistical support to keep your projects on track.