Anti-CCL27 Rabbit Polyclonal Antibody for WB & ELISA - Q9Y4X3

Core Product Specifications and Parameters

Biological Background: CCL27 Function and Localization

• C-C motif chemokine 27 (CCL27), also designated CTACK, ESkine, ILC, PESKY, SCYA27, or skinkine, is a small cytokine belonging to the CC chemokine family.
• It acts as a chemotactic factor that selectively attracts skin-associated memory T-lymphocytes, playing a key role in lymphocyte homing to cutaneous sites.
• CCL27 mediates its effects by binding to the chemokine receptor CCR10, triggering intracellular signaling cascades that promote cell migration.
• The protein is secreted and structurally characterized by a conserved chemokine fold with two disulfide bonds, as supported by its inclusion in the 3D-structure keyword.
• Tissue expression analysis shows CCL27 predominantly in testis, thymus, placenta, ovary, and skin, aligning with its function in cutaneous immunity.
• The UniProt-reviewed entry reports a molecular mass of 12,618 Da, while experimental detection often shows a band at approximately 13 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 25–112) excludes the signal peptide, targeting the mature secreted CCL27; this design may improve specificity for the functional form in biological fluids.
• When performing WB, note that the predicted molecular weight is ~12.6 kDa, but post-translational modifications or dimerization could yield bands at higher masses. Include appropriate positive controls such as skin or thymus lysates.
• For ELISA applications, consider using this polyclonal antibody as a capture reagent paired with a monoclonal detection antibody to enhance sensitivity and specificity. Optimize coating concentrations and blocking conditions empirically.
• This antibody recognizes both human and mouse CCL27 (as per cross-reactivity), but signal intensity may vary; validate cross-reactivity in your target species with appropriate controls.
• Due to the antibody’s polyclonal origin, batch-to-batch variability is possible; we recommend lot-specific validation for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your raw-material procurement by providing validated antibody pairs, recombinant proteins, and ancillary reagents for immunodiagnostic and research applications. For chemokine-oriented studies, we offer a comprehensive selection of anti-chemokine antibodies and antigens to accelerate your project from concept to clinic. Contact our team for bulk orders, custom sourcing, and technical consultation.