Anti-CCL5/RANTES Polyclonal Antibody for WB, IF-P, ELISA - P13501

Core Product Specifications and Parameters

Biological Background: CCL5/RANTES Function and Localization

• CCL5 (C-C motif chemokine 5), also known as RANTES, EoCP, SIS-delta, SCYA5, and TCP228, is a secreted chemokine belonging to the CC chemokine family.
• It functions as a chemoattractant for blood monocytes, memory T-helper cells, and eosinophils, and induces histamine release from basophils and eosinophil activation. Related references: PMID:1380064, PMID:15923218, PMID:16791620
• It is a major HIV-suppressive factor produced by CD8+ T-cells, with the processed form RANTES(3-68) acting as a more potent inhibitor of HIV-1 infection than the full-length protein. Related references: PMID:1380064, PMID:15923218, PMID:16791620
• CCL5 may act as an agonist of GPR75, stimulating inositol trisphosphate production and calcium mobilization, and potentially playing a role in neuron survival and insulin secretion. Related references: PMID:23979485
• The protein is expressed specifically in T-cells and macrophages, and has been detected at the protein level in follicular fluid.
• Post-translational modifications include glycosylation and the formation of disulfide bonds, and it contains a signal peptide for secretion.
• Its subcellular localization is primarily secreted.

Experimental Guidance and Technical Tips

• Since the immunogen covers the mature CCL5 sequence (a.a. 24-91), this antibody likely recognizes the mature secreted form.
• For Western blot, the predicted molecular weight is approximately 10 kDa; consider using a high-percentage gel and appropriate low-MW markers.
• The antibody is validated for human, mouse, and rat samples; validate in the relevant tissue or cell type.
• For ELISA, consider pairing with another CCL5 antibody if developing a sandwich assay; the polyclonal nature may provide good detection sensitivity.
• For immunofluorescence (IF-P), ensure proper fixation and permeabilization as CCL5 is secreted; it may be detected intracellularly in secreting cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high-quality anti-CCL5/RANTES polyclonal antibody for immunodetection applications, part of our comprehensive portfolio for inflammation, immune response, and HIV research. We provide validated antibody pairs, monoclonal and polyclonal options, and bulk ancillary reagents to support diagnostic development from concept to clinic. Contact us for tailored sourcing solutions.