Anti-CCT5 Polyclonal Antibody for WB, ELISA - P48643

Core Product Specifications and Parameters

Biological Background: CCT5 Function and Localization

• CCT5 encodes the epsilon subunit of the chaperonin-containing T-complex (TRiC), also known as CCT-epsilon or TCP-1-epsilon.
• As a component of the TRiC complex, CCT5 mediates ATP-dependent folding of actin, tubulin, and other client proteins, essential for cytoskeletal integrity (PubMed:25467444, PubMed:36493755, PubMed:35449234). Related references: PMID:25467444 PMID:36493755 PMID:35449234
• TRiC participates in WRAP53/TCAB1 folding, contributing to telomere maintenance (PubMed:25467444). Related references: PMID:25467444
• The complex is involved in BBSome assembly, a ciliogenesis-related vesicle transport pathway (PubMed:20080638). Related references: PMID:20080638
• CCT5 localizes to the cytoplasm, centrosome, and cytoskeleton, consistent with its chaperone role.
• Post-translational modifications include acetylation and phosphorylation; the protein contains ATP-binding and nucleotide-binding domains.
• Mutations in CCT5 are associated with hereditary sensory neuropathy, highlighting disease relevance.
• Alternative splicing produces multiple transcript variants, potentially affecting epitope recognition.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–270 of human CCT5 (N-terminal region); consider epitope accessibility in native versus denaturing conditions.
• Verify antibody reactivity in your specific sample system, as alternative splicing may affect isoform detection.
• This polyclonal antibody demonstrates cross-reactivity with human, mouse, and rat; validate with appropriate controls when using other species.
• For Western blotting, a band around 60 kDa is expected; optimize loading and transfer conditions for your model.
• In ELISA, consider using recombinant protein as antigen to titer specificity.

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