Anti-CCL3 Rabbit Polyclonal Antibody for WB, ELISA - P10147

Core Product Specifications and Parameters

Biological Background: CCL3 Function and Localization

• CCL3, also known as MIP-1-alpha or G0S19-1, is a small (10 kDa) secreted cytokine belonging to the CC chemokine family.
• It exhibits inflammatory and chemokinetic properties by binding to chemokine receptors CCR1, CCR4, and CCR5.
• CCL3 is a potent HIV-suppressive factor produced by CD8+ T-cells; recombinant MIP-1-alpha inhibits replication of HIV-1, HIV-2, and SIV in a dose-dependent manner.
• It contains structural features typical of chemokines, including conserved disulfide bonds and a signal peptide for secretion.
• Subcellularly, CCL3 is localized to the extracellular space upon secretion.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the mature human CCL3 protein (amino acids 24–92), which spans the core chemokine domain.
• For Western blotting, consider using low molecular weight markers due to the protein's small size (~10 kDa) and optimize transfer conditions for small proteins.
• In ELISA applications, this polyclonal antibody may be used for capture or detection in sandwich formats; validate performance using recombinant CCL3 standards and biological samples such as cell culture supernatants.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays.
• Cross-reactivity with mouse and rat CCL3 allows for multi-species studies, but species-specific sequence differences may necessitate validation in the target sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering a one-stop bridge from concept to clinic. This anti-CCL3 polyclonal antibody supports research in inflammation, chemokine signaling, and infectious disease. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development.