CCL25 Rabbit pAb for WB, ELISA - O15444

Core Product Specifications and Parameters

Biological Background: CCL25 Function and Localization

• CCL25, also known as TECK (Thymus-Expressed Chemokine), is a small cytokine belonging to the CC chemokine family, which plays a role in immune cell trafficking.
• The protein exists in at least two isoforms; isoform 1 acts as a chemoattractant via CCR9, while isoform 2 functions as an antagonist of isoform 1, modulating its activity.
• CCL25 is predominantly secreted and exhibits chemotactic activity toward thymocytes, macrophages, THP-1 cells, and dendritic cells, but is inactive on peripheral blood lymphocytes and neutrophils.
• It binds to the atypical chemokine receptor ACKR4, mediating the recruitment of beta-arrestin (ARRB1/2) to ACKR4, suggesting involvement in non-classical chemokine signaling.
• Expression is highly specific: CCL25 is found at high levels in the thymus and small intestine, and is particularly expressed by thymic dendritic cells.
• The protein is implicated in T-cell development and may contribute to inflammatory responses, consistent with its chemotactic and cytokine functions.
• Post-translational features include a signal peptide and disulfide bonds, characteristic of secreted chemokines, and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24-150 of human CCL25, covering the mature secreted protein; this design may favor detection of both isoform 1 and isoform 2 if they share the sequence.
• For Western blot, consider using reducing and non-reducing conditions, as disulfide bonds may influence migration; the predicted size is 17 kDa, but post-translational modifications could cause slight shifts.
• The antibody is raised in rabbit and may require appropriate anti-rabbit secondary detection reagents; validate in the relevant sample system (e.g., thymus or small intestine tissue lysates).
• In ELISA, use recombinant CCL25 as a positive control and optimize coating concentrations; cross-reactivity with other chemokines should be ruled out if multiplexing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable, one-stop access to IVD raw materials. For CCL25-related studies—whether investigating T-cell development, chemokine signaling, or inflammatory diseases—we can support your pipeline with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline procurement and accelerate your project from concept to clinic.