Anti-Human IL-5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05113

Core Product Specifications and Parameters

Biological Background: Interleukin-5 Function and Localization

• Interleukin-5 (IL-5) is a homodimeric cytokine, also known as eosinophil differentiation factor (EDF) and T-cell replacing factor (TRF), encoded by the IL5 gene (GeneID: 3567).
• Plays a critical role in the survival, differentiation, and chemotaxis of eosinophils. Related references: PMID:2653458 / PMID:9010276
• Promotes growth, differentiation, and immunoglobulin production in activated and resting B-cells.
• Binds to a heterodimeric receptor composed of IL5RA and CSF2RB subunits. Related references: PMID:1495999 / PMID:22528658
• Receptor engagement activates kinases including LYN, SYK, and JAK2, propagating signals through RAS-MAPK and JAK-STAT5 pathways. Related references: PMID:7613138
• Secreted glycoprotein with intramolecular disulfide bonds; present in peripheral blood mononuclear cells.
• Post-translational modifications include N-linked glycosylation and disulfide-bond formation essential for bioactivity.
• Alternative splicing generates multiple isoforms; the canonical form is a 134-amino acid precursor with a 19-amino acid signal peptide, yielding a mature protein of ~15 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–134) corresponds to the mature IL-5 protein; for Western blot, a band around 15 kDa under reducing conditions is expected; a ~30 kDa band may represent the covalent homodimer under non-reducing conditions.
• For immunofluorescence (IF/ICC), permeabilization may be required to detect intracellular or secreted IL-5; optimize fixation conditions.
• In ELISA, pilot experiments should determine optimal capture and detection conditions using recombinant IL-5 standards.
• Due to predicted wide cross-reactivity, this antibody may recognize IL-5 from multiple species; validate in the target species of interest.
• Store at recommended temperature and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from early-stage discovery to clinical application. This anti-human IL-5 polyclonal antibody is part of our extensive portfolio for immunology and inflammation research, backed by reliable supply chains and technical consultation. We provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.