Anti-COL4A5 Polyclonal Antibody for WB, IF/ICC, ELISA - P29400

Core Product Specifications and Parameters

Biological Background: COL4A5 Function and Localization

• Type IV collagen alpha-5 chain functions as the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork with laminins, proteoglycans, and entactin/nidogen.
• The protein is secreted and localizes to the extracellular space, extracellular matrix, and basement membranes.
• Isoform 2 exhibits tissue-specific expression in the kidney.
• COL4A5 is implicated in Alport syndrome, a genetic disorder characterized by progressive kidney disease, sensorineural deafness, and ocular abnormalities.
• The protein undergoes post-translational modifications, including hydroxylation and glycosylation, and forms disulfide bonds.
• Contains repetitive collagenous domains typical of type IV collagens, contributing to the formation of triple-helical structures.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1462-1691) of human COL4A5, which lies within the NC1 domain. This domain is involved in protomer assembly and may be well-presented in native basement membrane extracts.
• For Western blotting, the predicted molecular weight is approximately 161 kDa. Use a low-percentage polyacrylamide gel (e.g., 6-8%) to facilitate transfer of this high-molecular-weight protein, and consider extended transfer times.
• In immunofluorescence/immunocytochemistry, COL4A5 localizes to basement membranes. For optimal staining, use frozen or paraffin-embedded tissue sections. Antigen retrieval methods may be required for paraffin sections; consult the literature for specific protocols.
• ELISA applications may benefit from using recombinant COL4A5 protein or basement membrane extracts as coating antigen. Validate antibody specificity with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a unified sourcing platform for IVD raw materials, including this anti-COL4A5 polyclonal antibody tailored for glomerular basement membrane investigations. Our comprehensive supply chain covers validated antibody pairs, bulk ancillary reagents, and rare-target raw materials, supporting your workflow from early-stage research to clinical applications. Contact us to explore custom sourcing and technical support for your specific needs.