Anti-CCL3L1 Rabbit Polyclonal Antibody for WB, ELISA - P16619

Core Product Specifications and Parameters

Biological Background: CCL3L1 Function and Localization

• CCL3L1, also known as LD78-beta, G0S19-2, or SCYA3L1, is a member of the CC chemokine family that is structurally related to CCL3/MIP-1alpha.
• The full-length protein is processed into a mature secreted form, LD78-beta(1-70), and further N-terminal truncation yields LD78-beta(3-70), which exhibits 20- to 30-fold higher chemotactic activity.
• CCL3L1 acts as a chemoattractant for lymphocytes and monocytes and signals through the chemokine receptors CCR1, CCR3, and CCR5.
• It functions as a potent inhibitor of HIV-1 infection, with the LD78-beta(3-70) variant showing enhanced antiviral activity, likely by competing with the virus for CCR5 co-receptor binding.
• The protein is secreted and contains a signal peptide that is cleaved during maturation.
• CCL3L1 is located on chromosome 17 within a chemokine gene cluster and exists in multiple copy number variations, with CCL3L3 representing a closely related gene.
• Post-translational modifications include disulfide bonds essential for the chemokine fold, and the protein has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 10 kDa; use high-percentage gels (e.g., 15% SDS-PAGE) and low pore-size membranes to retain small proteins.
• Recombinant protein immunogen based on amino acids 24-93 suggests recognition of the mature secreted form; precursor and cleaved variants may also be detected if present.
• In ELISA applications, coat recombinant CCL3L1 or native protein from relevant biological fluids and consider using a matched antibody pair for sandwich assays.
• Because CCL3L1 shares high sequence homology with CCL3 and CCL3L3, verify specificity using knockout cells or specific recombinant antigens if cross-reactivity is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and researchers with streamlined access to validated IVD raw materials. This anti-CCL3L1 polyclonal antibody supports studies in chemokine biology and HIV research, complementing our broader portfolio of optimized antibodies, bulk ancillary reagents, and rare-target sourcing solutions. From concept to clinic, we bridge your supply needs with high-quality immunoreagents and tailored technical support.