Anti-Arp2 Rabbit Monoclonal Antibody for WB and ELISA - P61160

Core Product Specifications and Parameters

Biological Background: Arp2 Function and Localization

• Arp2, also known as Actin-like protein 2, is an ATP-binding component of the Arp2/3 complex, a key mediator of actin polymerization upon nucleation-promoting factor (NPF) stimulation. Related references: PMID:9000076
• The Arp2/3 complex drives the formation of branched actin networks in the cytoplasm, providing force for cell motility, and Arp2 contacts the pointed end of the daughter filament. Related references: PMID:9000076
• In podocytes, Arp2 is essential for lamellipodia formation, acting downstream of AVIL and PLCE1 regulation. Related references: PMID:29058690
• Nuclear Arp2/3 complex promotes actin polymerization, contributing to gene transcription regulation and DNA damage repair. Related references: PMID:17220302 PMID:29925947
• For homologous recombination repair, nuclear Arp2/3 complex drives the motility of double-strand breaks. Related references: PMID:29925947
• Arp2 localizes to the cytoplasm, cytoskeleton, cell projections, and nucleus.
• Post-translational modifications include acetylation, and Arp2 undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 295-394 of human Arp2 (P61160); for ortholog detection, verify sequence conservation in mouse and rat.
• For Western blot, consider an expected band near 45 kDa and include appropriate positive controls from human, mouse, or rat cell lysates.
• In ELISA, validate with recombinant or native Arp2 protein, and optimize coating and detection conditions.
• Cross-reactivity is reported for human, mouse, and rat; however, confirm specificity in your target species with blocking peptides or knockout validation.
• For applications not listed (e.g., IF, IP), pilot testing is recommended due to potential epitope accessibility differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, accelerating projects from concept to clinic. This anti-Arp2 monoclonal antibody supports research in actin cytoskeleton dynamics, cell motility, and DNA repair pathways. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development.