Anti-TARBP2 Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q15633

Core Product Specifications and Parameters

Biological Background: TARBP2 Function and Localization

• TARBP2 is an essential subunit of the RISC loading complex, where it partners with DICER1 and AGO2 to cleave precursor microRNAs (pre-miRNAs) into mature miRNAs and load them onto AGO2, forming the core of the RNA-induced silencing complex (RISC). It is also implicated in the biogenesis of siRNAs from double-stranded RNA. Related references: PMID:15973356, PMID:16142218, PMID:16271387
• TARBP2 can modulate DICER1 cleavage site selection on certain pre-miRNAs, influencing mature miRNA sequence and function. Related references: PMID:23063653
• TARBP2 acts as a negative regulator of antiviral innate immunity by inhibiting IRF7 phosphorylation and promoting its ubiquitin-mediated degradation, thereby suppressing IFN-β signaling. Related references: PMID:30927622
• During HIV-1 infection, TARBP2 binds the viral TAR RNA element to enhance translation of viral transcripts, partly by inhibiting the antiviral kinase PKR/EIF2AK2. It also recruits the methyltransferase FTSJ3 to the HIV-1 genome for 2'-O-methylation, facilitating immune evasion. Related references: PMID:11438532, PMID:12475984, PMID:2011739, PMID:30626973
• TARBP2 localizes predominantly to the cytoplasm and perinuclear region, with detectable nuclear localization, and its activity is regulated by phosphorylation.
• The protein contains double-stranded RNA-binding domains (dsRBDs) that mediate interactions with RNA and protein partners, and its expression is controlled by alternative promoter usage.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 200-300) is conserved across human, mouse, and rat TARBP2, supporting the antibody’s reported cross-reactivity in Western blot, immunohistochemistry, and immunofluorescence.
• For Western blot analysis, expect a specific band around 39 kDa; optimize protein extraction and loading conditions to achieve clear resolution.
• When using for IHC or IF, validate in appropriate positive control tissues or cell lines expressing TARBP2, and include proper fixation and antigen retrieval steps.
• Given TARBP2’s involvement in RNA interference and viral infection, this antibody may be useful for investigating miRNA/siRNA pathways or HIV-host interactions; empirical optimization of detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing platform for IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This TARBP2 antibody supports studies in RNA interference, antiviral immunity, and HIV biology. Our supply network ensures consistent access to high-quality monoclonal antibodies and customizable bulk orders to accelerate your assay development.