Anti-TAP2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q03519

Core Product Specifications and Parameters

Biological Background: TAP2 Function and Localization

• TAP2 is an ABC transporter that, in complex with TAP1, mediates the unidirectional translocation of peptide antigens from the cytosol to the ER for loading onto MHC class I molecules (PubMed:25377891, PubMed:25656091). Related references: PMID:25377891 PMID:25656091
• During the transport cycle, TAP2 alternates between inward-facing (cytosolic peptide binding) and outward-facing (ER lumen peptide release) conformations. ATP binding and hydrolysis drive these conformational changes (PubMed:11274390, PubMed:25377891, PubMed:25656091). Related references: PMID:11274390 PMID:25377891 PMID:25656091
• TAP2 preferentially transports intracellular peptides of 8-13 amino acids, selecting for hydrophobic or charged C-terminal residues and a hydrophobic residue at position 3, while proline at position 2 is destabilizing (PubMed:11274390, PubMed:7500034, PubMed:9256420). Related references: PMID:11274390 PMID:7500034 PMID:9256420
• As part of the peptide loading complex, TAP2 acts as a molecular scaffold essential for peptide-MHC I assembly and stable antigen presentation (PubMed:1538751, PubMed:25377891, PubMed:26611325). Related references: PMID:1538751 PMID:25377891 PMID:26611325
• The protein is localized to the endoplasmic reticulum membrane.
• TAP2 contains ATP-binding domains, transmembrane helices, and metal-binding sites (magnesium), and is involved in adaptive immunity and host-virus interactions.
• Alternative splicing of the TAP2 gene produces multiple isoforms, contributing to functional diversity in peptide transport.

Experimental Guidance and Technical Tips

• For western blotting, a band at approximately 76 kDa is expected for endogenous TAP2. Use standard SDS-PAGE conditions and confirm specificity with a loading control or knockdown/knockout validation.
• In immunofluorescence, TAP2 staining typically shows a perinuclear reticular pattern consistent with ER localization. Validate with an ER marker co-stain and siRNA-mediated knockdown.
• For ELISA applications, this antibody can serve as a capture or detection reagent. Optimal pairing should be determined empirically with a complementary antibody targeting a distinct TAP2 epitope.
• The immunogen (amino acids 450–686) corresponds to the cytoplasmic nucleotide-binding domain, a region highly conserved between human and mouse, which likely accounts for the observed cross-reactivity.
• This antibody has not been tested in IHC or flow cytometry; if these applications are needed, titrate and validate under appropriate fixation/permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we empower diagnostic manufacturers and research labs with a complete range of IVD raw materials and customized sourcing services—from validated antibody pairs and monoclonal antibodies to bulk reagents and rare target procurement. Our TAP2 Rabbit mAb is an ideal choice for studies on antigen processing and MHC class I presentation, and our team stands ready to support your project with technical consultation and tailored raw-material solutions.