Anti-Arp3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61158

Core Product Specifications and Parameters

Biological Background: Arp3 Function and Localization

• Arp3 (Actin-related protein 3), also known as Actin-like protein 3, is encoded by the ACTR3 gene and serves as an ATP-binding subunit of the Arp2/3 complex.
• The Arp2/3 complex mediates actin polymerization upon stimulation by nucleation-promoting factors (NPFs), generating branched actin networks that provide mechanical force for cell motility. Related references: PMID:9000076
• Arp3 is predominantly localized in the cytoplasm and cytoskeleton, particularly at cell projections such as lamellipodia, but also localizes to the nucleus for specialized functions.
• In podocytes, Arp3 is essential for the formation of lamellipodia downstream of AVIL and PLCE1 regulatory pathways. Related references: PMID:29058690
• Within the nucleus, the Arp2/3 complex drives actin polymerization to regulate gene transcription and facilitates homologous recombination repair of DNA double-strand breaks by promoting mobility of damage sites. Related references: PMID:17220302, PMID:29925947
• Arp3 contributes to ciliogenesis, underscoring its importance in the formation of primary cilia. Related references: PMID:20393563
• The protein undergoes post-translational modifications including acetylation, and is classified by keywords such as actin-binding, nucleotide-binding, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1-418 of human Arp3 (full-length), suggesting the antibody may recognize the native protein across multiple species provided sequence homology is sufficient.
• For Western blot, human and mouse cell lysates are appropriate due to the antibody's known cross-reactivity; a specific band near 47 kDa should be expected.
• In immunofluorescence/ICC experiments, optimize fixation and permeabilization protocols to visualize Arp3 localization at the cytoskeleton, lamellipodia, and nucleus.
• ELISA performance may be enhanced by using the recombinant protein as a positive control; dilute samples appropriately to avoid hook effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers and research labs with high-quality antibodies like the Arp3 Rabbit pAb. For projects involving cytoskeleton dynamics, cell motility, or DNA repair pathways, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, while also facilitating custom orders for rare or emerging targets. Our integrated approach streamlines procurement from discovery to clinical application.