Anti-ARPC2 Monoclonal Antibody for WB - O15144

Core Product Specifications and Parameters

Biological Background: ARPC2 Function and Localization

• ARPC2 (Actin-related protein 2/3 complex subunit 2), also known as p34-ARC, is a 34 kDa subunit of the Arp2/3 complex, a key regulator of actin dynamics.
• The Arp2/3 complex mediates actin polymerization upon stimulation by nucleation-promoting factors (NPFs), generating branched actin networks that provide force for cell motility.
  Related references:
  PMID:9230079
• ARPC2 is thought to contact the mother actin filament, stabilizing the branch junction.
  Related references:
  PMID:9230079
• Beyond cytoskeletal functions, ARPC2 and the Arp2/3 complex promote actin polymerization in the nucleus, where they regulate gene transcription and DNA damage repair.
  Related references:
  PMID:29925947
• In response to DNA damage, nuclear Arp2/3 complex drives the motility of double-strand breaks, facilitating homologous recombination repair.
  Related references:
  PMID:29925947
• Subcellularly, ARPC2 localizes to the cytoplasm, cytoskeleton, cell projections, synapse, synaptosome, and nucleus.
• ARPC2 is subject to acetylation, and its three-dimensional structure has been determined, providing insights into its function.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 200–300 of human ARPC2. This region is predicted to be surface-exposed and specific to ARPC2, which may contribute to antibody specificity.
• For Western blotting, the antibody detects a band consistent with the predicted molecular weight of ~34 kDa. Use appropriate controls to confirm target identity.
• In immunofluorescence applications, ARPC2 is expected to localize to both the cytoplasm and nucleus, with enrichment at cell protrusions and synaptic structures.
• For immunoprecipitation, this antibody can be used to pull down the Arp2/3 complex; co-immunoprecipitation of other complex subunits (e.g., ARPC1, ARPC3) can serve as a validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibodies, bulk ancillary reagents, and tailor-made rare-target procurement. This anti-ARPC2 antibody addresses the growing demand for cytoskeleton and DNA repair research reagents, backed by CamelBio's expertise in consolidated supply chain management and technical guidance.