Anti-AGR2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O95994

Core Product Specifications and Parameters

Biological Background: Anterior gradient protein 2 homolog Function and Localization

• AGR2 (Anterior gradient protein 2 homolog) is a human protein encoded by the AGR2 gene and belongs to the protein disulfide isomerase family.
• It is required for MUC2 post-transcriptional synthesis and secretion, playing a critical role in mucus production by intestinal cells (By similarity).
• AGR2 acts as a proto-oncogene involved in cell migration, differentiation, and growth, and promotes cell adhesion. Related references: PMID:23274113
• The protein localizes to the endoplasmic reticulum and is secreted, aligning with its function in secretory pathways.
• AGR2 exhibits strong expression in trachea, lung, stomach, colon, prostate, and small intestine, and is weakly expressed in many other tissues and ER-positive breast cancer cell lines.
• It contains a signal peptide and disulfide bonds, features important for its secretion and structural integrity.
• AGR2 has been characterized by proteomics and direct protein sequencing, and disease variants have been reported.

Experimental Guidance and Technical Tips

• For Western blotting, use standard protocols with appropriate blocking agents; optimization of antibody dilution and sample preparation is recommended for each target tissue.
• In immunofluorescence and ICC, consider permeabilization steps to detect endoplasmic reticulum-localized AGR2, and validate fixation conditions to preserve epitope accessibility.
• Given the immunogen spans a conserved N-terminal region, cross-reactivity with mouse AGR2 is expected; validate in mouse-derived samples as needed.
• Peptide blocking experiments using the immunogen sequence can be performed to confirm antibody specificity.
• For ELISA, test various coating conditions and antibody concentrations, and include appropriate controls to ensure robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive, one-stop access to IVD raw materials, technical services, and consulting. Supporting AGR2-related research and assay development, we offer validated monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to streamline your workflow from concept to clinic. Our sourcing capabilities extend to rare targets and custom development, ensuring you find the right raw materials for your specific diagnostic needs.