Anti-RARα Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P10276

Core Product Specifications and Parameters

Biological Background: RARα Function and Localization

• RARA encodes retinoic acid receptor alpha (RARα), also known as NR1B1, a nuclear receptor that functions as a ligand-dependent transcription factor.
• RARα binds all-trans and 9-cis retinoic acid and forms heterodimers with retinoid X receptors (RXRs) to recognize retinoic acid response elements (RAREs) in target gene promoters (PubMed:19850744, PubMed:20215566, PubMed:19398580). Related references: PMID:19850744 PMID:20215566 PMID:19398580
• In the absence of ligand, the RXR-RAR heterodimer recruits corepressor complexes, leading to histone deacetylation and transcriptional repression (PubMed:16417524). Related references: PMID:16417524
• Ligand binding triggers corepressor dissociation and coactivator recruitment, resulting in transcriptional activation of target genes (PubMed:19850744, PubMed:20215566, PubMed:37478846). Related references: PMID:19850744 PMID:20215566 PMID:37478846
• RARα plays critical roles in spermatogenesis, skeletal growth, and vascular endothelial responses to shear stress (PubMed:28167758). Related references: PMID:28167758
• The protein localizes to both the nucleus and cytoplasm, consistent with its dual roles in transcription and non-genomic signaling.
• RARα is expressed in monocytes and is classified as a proto-oncogene; chromosomal rearrangements involving RARA are a hallmark of acute promyelocytic leukemia (APL).
• Post-translational modifications include phosphorylation, ubiquitination, and isopeptide bond formation; the protein contains zinc-finger DNA-binding domains.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the N-terminal region (amino acids 1-100) of human RARα. For western blotting, a band around 51 kDa is expected; consider using appropriate positive controls (e.g., APL cell lines like NB4) and validating with siRNA knockdown if specificity confirmation is needed.
• The antibody is classified for WB, IHC-P, and ELISA. For IHC-P, antigen retrieval conditions may need optimization; a standard citrate or EDTA-based heat retrieval is suggested. In ELISA, pair with a detection antibody recognizing a non-overlapping epitope if used in sandwich format.
• Cross-reactivity with mouse and rat is reported, but users should verify performance in their specific tissue or cell models due to potential species-specific differences in expression or post-translational modifications.
• As RARα can shuttle between nucleus and cytoplasm, subcellular fractionation or immunofluorescence may complement localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials like this Anti-RARα rabbit monoclonal antibody, supporting studies on retinoic acid signaling, cancer, and developmental biology. In addition to validated primary antibodies, we offer optimized monoclonal and polyclonal pairs, bulk ancillary reagents, and specialized sourcing for rare-target raw materials—streamlining your workflow from discovery to clinical application. Partner with CamelBio to efficiently bridge the gap between concept and clinic.