Anti-APP (Amyloid-beta precursor protein) Rabbit Monoclonal Antibody [KO Validated] for WB, IP, IF, IHC-P, ELISA - P05067

Core Product Specifications and Parameters

Biological Background: APP Function and Localization

• APP is a cell surface receptor that promotes neurite growth, neuronal adhesion, and synaptogenesis through homophilic interactions (PubMed:25122912). Related references: PMID:25122912
• It functions as a kinesin I membrane receptor, mediating axonal anterograde transport of beta-secretase and presenilin 1 towards synapses (PubMed:17062754, PubMed:23011729). Related references: PMID:17062754 | PMID:23011729
• APP is involved in copper homeostasis and oxidative stress; copper-metallated APP can induce neuronal death or potentiate Cu2+-mediated low-density lipoprotein oxidation.
• Amyloid-beta peptides derived from APP are lipophilic metal chelators that bind copper, zinc, and iron, promote tau aggregation, and contribute to oxidative stress and neuroinflammation.
• Alternative splicing generates isoforms such as APP695 (predominant in neuronal tissue), APP751, and APP770 (non-neuronal); isoform APP751 is abundant in T-lymphocytes, and appican is expressed in astrocytes.
• Subcellular localization includes cell membrane, clathrin-coated pits, early endosomes, Golgi apparatus, endoplasmic reticulum, nucleus, and cell projections such as growth cones; secreted forms are also detected.
• Post-translational modifications include phosphorylation, glycosylation, disulfide bond formation, and ubiquitination; some isoforms possess a BPTI domain with serine protease inhibitor activity.
• Keywords from UniProt include Alzheimer disease, amyloidosis, apoptosis, cell adhesion, copper, iron, zinc binding, neurodegeneration, Notch signaling pathway, serine protease inhibitor, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 671-770 of human APP, encompassing the amyloid-beta sequence; the antibody may detect full-length APP and C-terminal fragments containing this region.
• For WB, consider validating the antibody using human, mouse, or rat brain lysates or APP-expressing cell lines; an ~87 kDa band is expected for full-length APP.
• The antibody is KO-validated, making it suitable for confirming APP knockout in cell lines via WB.
• For IHC-P, optimize antigen retrieval and include appropriate controls; signal may vary with tissue fixation and processing.
• In IF/ICC, expect membrane and intracellular punctate staining; use appropriate permeabilization and blocking steps.
• The antibody's reported reactivity in ELISA suggests potential for quantitative detection of APP in serum, CSF, or recombinant protein assays; pilot testing with your specific antigen format is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research labs from discovery to clinical application. This high-quality APP antibody, alongside validated antibody pairs, recombinant APP proteins, and bulk ancillary reagents for Alzheimer's disease biomarker development, can be procured through CamelBio's integrated supply network. For rare target antibodies or custom bulk orders, rely on CamelBio to bridge your concept-to-clinic requirements with dependable B2B service.