Anti-Arp3 Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - P61158

Core Product Specifications and Parameters

Biological Background: Arp3 Function and Localization

• Arp3 (Actin-related protein 3, ACTR3) is an ATP-binding component of the Arp2/3 complex, which mediates actin polymerization to form branched actin networks, providing the force for cell motility.
  Related references: PMID:9000076
• In podocytes, Arp3 is required for lamellipodia formation downstream of AVIL and PLCE1 regulation.
  Related references: PMID:29058690
• In the nucleus, the Arp2/3 complex promotes actin polymerization to regulate gene transcription and repair damaged DNA, including homologous recombination repair by driving motility of double-strand breaks.
  Related references: PMID:17220302 PMID:29925947
• Plays a role in ciliogenesis.
  Related references: PMID:20393563
• Subcellular localization includes cytoplasm, cytoskeleton, cell projections, and nucleus.
• Post-translational modifications include acetylation.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide encompassing amino acids 319–418 within the internal region of human Arp3. This design targets a conserved sequence, which may contribute to the observed cross-reactivity with mouse and rat.
• For Western blotting, the expected molecular weight is ~47 kDa. Use appropriate loading controls and optimize antibody dilution for your specific samples.
• For IHC-P and IF/ICC, ensure proper fixation and permeabilization protocols; paraffin-embedded sections may require antigen retrieval.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency. Validate in the relevant sample system, as performance may vary depending on experimental conditions.
• The listed applications are based on supplier data; verify each application independently when using this product in diagnostic or research workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This high-quality anti-Arp3 rabbit monoclonal antibody is suitable for studies on actin dynamics, cell motility, and nuclear functions. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials to streamline your assay development and production needs.