Anti-FAP (Fibroblast activation protein-α) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q12884

Core Product Specifications and Parameters

Biological Background: FAP Function and Localization

• FAP is a multifunctional cell surface serine protease, also known as seprase, exhibiting both post-prolyl endopeptidase and dipeptidyl peptidase activities, with a preference for cleaving substrates after proline residues. Related references: PMID:14751930, PMID:16223769, PMID:16410248.
• It degrades gelatin and heat-denatured type I collagen, contributing to extracellular matrix (ECM) remodeling during processes such as wound healing, fibrosis, and tumor invasion. Related references: PMID:10347120, PMID:10455171, PMID:12376466.
• FAP possesses dipeptidyl peptidase activity, cleaving N-terminal dipeptides after proline, and can process neuropeptide Y, peptide YY, substance P, and BNP32 as natural substrates. Related references: PMID:10347120, PMID:10593948, PMID:16175601, PMID:21314817.
• Located at the cell surface, particularly in lamellipodia, invadopodia, and ruffles, FAP is also secreted and found in the cytoplasm, facilitating local pericellular proteolysis.
• It is highly expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue, cirrhotic liver myofibroblasts, and glioma cells, but absent in normal adult tissues, making it a marker of activated fibroblasts in pathologic conditions.
• FAP promotes tumor growth by enhancing angiogenesis, degrading collagen fibers, suppressing immune cell antitumor responses, and modulating apoptosis, while paradoxically acting as a tumor suppressor in melanocytic cells in a protease-independent manner.
• As a glycoprotein with a predicted molecular weight of ~88 kDa (full-length), FAP includes isoforms from alternative splicing and is a member of the prolyl oligopeptidase family of serine proteases.

Experimental Guidance and Technical Tips

• The immunogen spans the catalytic domain (aa 411–510), suggesting the antibody may detect both membrane-bound and soluble FAP forms; verify in the relevant sample system.
• For western blotting, anticipate bands at ~87 kDa (full-length) and possibly ~26 kDa (alternatively spliced isoform or cleavage product); use appropriate loading controls and molecular weight markers.
• In IF/ICC applications, signal is expected at cell surface projections (lamellipodia, invadopodia); consider permeabilization to enhance accessibility to intracellular epitopes.
• Cross-reactivity with mouse and rat is reported; validate staining patterns in activated fibroblast or tumor cell lines from these species.
• For ELISA, recombinant human FAP protein or cell lysates from FAP-expressing lines may serve as capture reagents; pair with a compatible detection antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw-material solutions for IVD manufacturers and research laboratories, covering the entire product development pipeline. For targets like FAP—central to ECM remodeling and tumor microenvironment studies—we offer validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare or challenging targets. Partner with CamelBio to streamline your procurement and accelerate your diagnostic assay development.