Anti-A-RAF Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P10398

Core Product Specifications and Parameters

Biological Background: A-RAF Function and Localization

• Encoded by the ARAF gene, A-RAF is a proto-oncogene serine/threonine-protein kinase that belongs to the RAF family of kinases.
• Predominantly expressed in urogenital tissues.
• Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
• Regulates the TOR signaling cascade and phosphorylates PFKFB2. Related references: PMID:36402789
• Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest and expression of muscle-specific proteins.
• Undergoes alternative splicing, producing multiple isoforms with potential functional diversity.
• Classified as a phosphoprotein, its activity is modulated by phosphorylation; also contains a zinc-finger domain and an ATP-binding site.

Experimental Guidance and Technical Tips

• The immunogen region (amino acids 166–290) maps to the N‑terminal regulatory domain; consider this when designing experiments or interpreting results.
• For Western blot, the predicted molecular weight is ~68 kDa; validate band size in the relevant sample system and include appropriate controls.
• In IHC‑P, optimization of antigen retrieval and antibody dilution is recommended; human urogenital tissues can serve as positive controls based on tissue specificity.
• For ELISA, the recombinant immunogen may be used as a reference standard or coating antigen; include proper negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide streamlined access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For A-RAF-related studies in kinase signaling and cancer biology, we offer reliable sourcing support and technical consultation, helping you maintain a smooth supply chain from concept to clinical development.