Anti-ROR2 Rabbit Monoclonal Antibody for WB and ELISA - Q01974

Core Product Specifications and Parameters

Biological Background: ROR2 Function and Localization

• ROR2 (Tyrosine-protein kinase transmembrane receptor ROR2), also known as Neurotrophic tyrosine kinase, receptor-related 2 (NTRKR2) and encoded by the ROR2 gene, is a receptor that may play a role in chondrogenesis and skeletal development.
• It is required for cartilage and growth plate development, suggesting involvement in the early formation of chondrocytes.
• ROR2 phosphorylates YWHAB, which leads to the induction of osteogenesis and bone formation. Related references: PMID:17717073
• It may act as a receptor for Wnt ligand WNT5A, potentially leading to the inhibition of WNT3A-mediated signaling. Related references: PMID:25029443
• Despite its classification as a tyrosine kinase, ROR2 has been reported to have very little tyrosine kinase activity in vitro.
• The protein localizes to the cell membrane and contains domains such as immunoglobulin-like, kringle, and a tyrosine kinase domain, with post-translational modifications including sulfation, methylation, and disulfide bonds.
• It is associated with the Wnt signaling pathway and is classified under developmental proteins; mutations in this gene are linked to dwarfism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human ROR2, which corresponds to the N-terminal extracellular region; this may yield an antibody recognizing the full-length receptor. For Western blotting, expect a band at approximately 105 kDa; include appropriate positive and negative controls, and optimize blocking and detection conditions for your specific sample type.
• This rabbit monoclonal antibody is recommended for WB and ELISA; cross-reactivity with mouse has been noted, allowing potential use in mouse models. Validate detection specificity in the relevant species and tissue context.
• For ELISA, consider using the immunogen peptide or recombinant ROR2 protein as a coating antigen to establish standard curves and ensure assay linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw material sourcing, from concept to clinic. For researchers and diagnostic developers working on skeletal development, osteogenesis, or Wnt signaling, we supply validated antibodies like this anti-ROR2 monoclonal, along with optimized antibody pairs, bulk ancillary reagents, and rare target raw materials. Our procurement network ensures seamless access to high-quality reagents tailored to your assay development needs.