Anti-AHR Rabbit Monoclonal Antibody for WB, ELISA - P35869

Core Product Specifications and Parameters

Biological Background: AHR Function and Localization

• AHR (aryl hydrocarbon receptor) is a ligand-activated transcription factor of the basic helix-loop-helix (bHLH) family (class E bHLHe76) that senses environmental, dietary, microbiome, and metabolic compounds, playing critical roles in development, immunity, and cancer.
  Related references: PMID:23275542 PMID:30373764 PMID:32818467
• Upon ligand activation, AHR translocates from the cytoplasm to the nucleus, heterodimerizes with ARNT, and binds to xenobiotic response elements (XRE) to drive transcription of target genes, including the xenobiotic-metabolizing enzyme CYP1A1.
  Related references: PMID:23275542 PMID:30373764 PMID:7961644 PMID:33193710
• AHR mediates the biochemical and toxic effects of halogenated aromatic hydrocarbons and regulates angiogenesis, hematopoiesis, drug/lipid metabolism, cell motility, and immune modulation.
  Related references: PMID:12213388 PMID:34521881 PMID:7961644
• Endogenous ligands, particularly tryptophan derivatives such as indoles and kynurenic acid, activate AHR to suppress adaptive anti-tumor immunity and promote cancer cell motility.
  Related references: PMID:32818467 PMID:32866000
• AHR regulates the circadian clock by inhibiting CLOCK-BMAL1-mediated transcriptional activation of PER1, thereby repressing the basal and circadian expression of PER1.
  Related references: PMID:28602820
• AHR is widely expressed across human tissues, including blood, brain, heart, kidney, liver, lung, pancreas, skeletal muscle, and retinal photoreceptors.
  Related references: PMID:29726989
• Subcellular localization: AHR is primarily cytoplasmic under basal conditions but translocates to the nucleus upon ligand activation.
• Post-translational modifications: AHR is subject to acetylation and ADP-ribosylation, which modulate its transcriptional activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 619-848 at the C-terminal region of human AHR; the antibody may recognize both full-length AHR and C-terminal fragments.
• For Western blot analysis, a primary band near 96 kDa is expected. Validate reactivity in the relevant human sample system using known positive and negative controls.
• For ELISA applications, consider pairing this antibody with another monoclonal antibody targeting a distinct epitope to develop a sandwich ELISA. Optimize concentrations and dilutions experimentally.
• Cross-reactivity is reported for human AHR; if working with other species, verify cross-reactivity empirically due to potential sequence variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and researchers from concept to clinic. This AHR monoclonal antibody exemplifies our capability to supply high-quality reagents for transcription factor and xenobiotic response research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and sourcing services for rare targets, ensuring streamlined procurement for your assay development and production needs.