Anti-ARF1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P84077

Core Product Specifications and Parameters

Biological Background: ARF1 Function and Localization

• Functions as a small GTPase that regulates vesicle budding and uncoating within the Golgi complex by recruiting coatomer proteins in its GTP-bound form and mediating GTP hydrolysis-dependent coat dissociation (PubMed:8253837). Related references: PMID:8253837
• Interacts with PICK1 to limit inhibition of Arp2/3 complex activity, thereby modulating AMPA receptor trafficking, synaptic plasticity of excitatory synapses, and spine shrinkage during long-term depression (By similarity).
• Plays a key role in maintaining intestinal homeostasis by regulating intestinal stem cells and gut microbiota (By similarity).
• Critical for mast cell expansion by facilitating optimal mTORC1 activation, but not required for mast cell maturation (By similarity).
• Acts as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase, during microbial infection.
• Localizes to the Golgi apparatus membrane, synapse, synaptosome, and postsynaptic density.
• Post-translationally modified by N‑terminal myristoylation and acetylation; associated with disease variants, ER‑Golgi transport, and neuron‑related processes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 70–150 of human ARF1. This design may recognize both unmodified and post‑translationally modified forms within this region; validate in the relevant sample system.
• For Western blotting, the predicted band size is approximately 21 kDa. Include appropriate positive and negative controls to confirm specificity.
• For immunofluorescence/ICC, expect a Golgi‑associated punctate staining pattern; optimize fixation and permeabilization protocols to preserve organelle morphology.
• In ELISA, the peptide‑based immunogen typically ensures strong target binding; however, consider using recombinant ARF1 protein as a standard for accurate quantification.
• Cross‑reactivity is reported for human, mouse, and rat; verify performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one‑stop access to validated IVD raw materials, including optimized monoclonal antibodies like this anti-ARF1 clone. Our portfolio supports key research areas such as Golgi trafficking, synaptic biology, and infectious disease mechanisms. To accelerate your assay development, we also offer bulk ancillary reagents and customized sourcing for rare or difficult‑to‑obtain targets.