Anti-ARFRP1 / ARP Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q13795

Core Product Specifications and Parameters

Biological Background: ARFRP1/ARP Function and Localization

• ADP-ribosylation factor-related protein 1 (ARFRP1, also known as ARP, Arp1, ARL18) is a trans-Golgi-associated GTPase encoded by the ARFRP1 gene.
• It regulates protein sorting by controlling the targeting of ARL1 and its effector to the trans-Golgi network.
• ARFRP1 is required for the lipidation of chylomicrons in the intestine and VLDL lipidation in the liver, playing a role in lipid metabolism.
• It localizes to the Golgi apparatus, primarily at the trans-Golgi network.
• Belongs to the small GTPase superfamily, binding GTP and functioning as a molecular switch.
• Expressed in most tissues (ubiquitous).
• Keywords include Acetylation and Alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 102–201 of human ARFRP1, targeting the native protein. Consider this region when designing downstream experiments.
• Recommended applications include WB, IF/ICC, and ELISA. For WB, the predicted band is at ~23 kDa; validate detection in your sample system.
• The antibody cross-reacts with human, mouse, and rat, suggesting it targets a conserved epitope. Verify reactivity in non-human samples if used.
• For IF/ICC, consider fixation and permeabilization conditions suitable for Golgi apparatus targets. Include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports researchers and IVD developers by providing this ARFRP1 rabbit monoclonal antibody alongside a full range of core raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. Our one-stop platform streamlines sourcing for rare and specialized targets, ensuring consistent supply for projects in cell biology, lipid metabolism, and Golgi trafficking research.