Anti-AKR1C3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P42330

Core Product Specifications and Parameters

Biological Background: AKR1C3 Function and Localization

• AKR1C3, also known as 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type II, DD3, PGFS, and other aliases, is encoded by the AKR1C3 gene in humans.
• It is a cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids with broad substrate specificity, primarily generating 17beta-hydroxysteroids.
  Related references: PMID:10998348, PMID:11165022, PMID:20036328
• AKR1C3 plays a central role in androgen biosynthesis, operating in both the classical (converting delta4-androstenedione to testosterone) and backdoor (reducing androsterone to 5alpha-androstane-3alpha,17beta-diol) pathways.
  Related references: PMID:10998348, PMID:11165022, PMID:20036328, PMID:10557352, PMID:9415401
• It reduces 5alpha-DHT to the less potent androgen 5alpha-androstane-3alpha,17beta-diol, potentially regulating androgen receptor signaling.
  Related references: PMID:10557352, PMID:10998348, PMID:11165022
• In estrogen metabolism, it converts estrone to the bioactive 17beta-estradiol.
  Related references: PMID:10998348, PMID:11165022, PMID:20036328
• AKR1C3 functions as a prostaglandin F2alpha synthase, reducing PGD2 and PGH2 to PGF2alpha and 11beta-PGF2alpha, respectively.
  Related references: PMID:10622721, PMID:11165022, PMID:15047184
• The protein also exhibits retinaldehyde reductase activity toward 9-cis-retinal and can reduce various carbonyl compounds in vitro.
  Related references: PMID:21851338
• AKR1C3 localizes to the cytoplasm and is expressed in many tissues, with high levels in prostate and mammary gland, making it relevant to hormone-dependent cancers.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 37 kDa is expected under reducing conditions. Adjust antibody dilution and blocking conditions according to standard protocols.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic staining. Fixation and permeabilization should be optimized for the target cell type.
• For immunoprecipitation, use suitable lysis buffers that preserve native protein conformation and interactions.
• The immunogen covers the full-length protein (amino acids 1-323), supporting detection of multiple isoforms; however, cross-reactivity with related AKR1C family members should be considered due to high homology.
• For ELISA applications, pair this antibody with an appropriate detection antibody for sandwich ELISA formats, verifying reactivity with native and recombinant antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-AKR1C3 antibody supports research into androgen metabolism and hormone-related diseases, complementing our full portfolio of diagnostic-grade raw materials and technical services from concept to clinic.