Anti-AKR1C1 Polyclonal Antibody for WB, ELISA - Q04828

Core Product Specifications and Parameters

Biological Background: AKR1C1 Function and Localization

• AKR1C1 (Aldo-keto reductase family 1 member C1) is also known as 20alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase 1, and high-affinity hepatic bile acid-binding protein, reflecting its multifunctional roles in steroid and xenobiotic metabolism.
• As a cytosolic NADPH-dependent oxidoreductase, AKR1C1 catalyzes the stereospecific reduction of ketosteroids to hydroxysteroids, preferentially generating 20alpha-hydroxysteroids, but also 3alpha/beta- and 17beta-hydroxysteroids, thus modulating the activity of various steroid hormones. Related references: PMID:10998348 PMID:11013348 PMID:14672942
• In neurosteroid metabolism, AKR1C1 converts 5alpha-dihydrodeoxycorticosterone into the neuroactive steroid 3alpha,5alpha-tetrahydrodeoxycorticosterone (allopregnanolone precursor), which potentiates GABA(A) receptors, and also inactivates 3alpha,5alpha-tetrahydroprogesterone, thereby influencing neuronal excitability. Related references: PMID:11995921 PMID:12604236
• AKR1C1 participates in androgen catabolism by acting as a phase I hydroxylase or in an alternative phase II pathway by directly reducing conjugated androgens, highlighting its flexibility in hormone clearance. Related references: PMID:19218247
• Subcellularly, AKR1C1 localizes predominantly to the cytosol, consistent with its role in intracellular steroid metabolism.
• The protein is widely expressed, with highest levels in liver, mammary gland, and brain, aligning with its roles in systemic steroid processing and neuroactive steroid synthesis.
• Classified under the aldo-keto reductase (AKR) superfamily, AKR1C1 is a member of the AKR1C subfamily, which includes closely related isoforms with overlapping yet distinct substrate specificities.

Experimental Guidance and Technical Tips

• The immunogen was a full-length recombinant human AKR1C1 protein (residues 1-323), which may confer recognition of native, denatured, and linear epitopes; consider optimizing conditions for each application.
• For Western blotting, a signal near 37 kDa is expected in human and mouse tissues; include appropriate positive and negative controls and validate band specificity using siRNA knockdown or knockout models where possible.
• In ELISA, the polyclonal nature may capture multiple epitopes, potentially increasing sensitivity; however, cross-reactivity with other AKR1C isoforms should be assessed if isoform-specific detection is required.
• For mouse samples, verify cross-reactivity experimentally, as sequence homology supports reactivity but individual experimental conditions may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting researchers and manufacturers working on steroid metabolism and neurological disorders. From validated antibody pairs to bulk ancillary reagents, we streamline procurement for projects targeting AKR1C1 and related pathways. Our one-stop sourcing model also accommodates custom rare-target raw material requests, enabling seamless progression from early R&D to clinical application.