Anti-ARID3A Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q99856

Core Product Specifications and Parameters

Biological Background: ARID3A Function and Localization

• ARID3A (AT-rich interactive domain-containing protein 3A), also known as Bright, DRIL1, DRIL3, and E2FBP1, is a transcription factor.
• It is involved in the control of cell cycle progression via the RB1/E2F1 pathway and plays a role in B-cell differentiation.
• The protein contains an ARID DNA-binding domain and localizes to both the nucleus and cytoplasm.
• ARID3A is widely expressed, with highest levels in skeletal muscle, thalamus, and colon.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (isopeptide bond), suggesting regulatory mechanisms.
• Keywords indicate its involvement in transcription regulation and DNA binding.

Experimental Guidance and Technical Tips

• For Western blot, the expected molecular weight is approximately 63 kDa; consider validating with appropriate positive and negative controls.
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval conditions and antibody dilution for your specific tissue type.
• The antibody is also suitable for immunoprecipitation and ELISA; verify performance under your experimental conditions.
• Cross-reactivity with mouse and rat allows use in common model organisms; confirm specificity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and personalized sourcing for rare targets. This ARID3A antibody supports research into B-cell differentiation and RB1/E2F1-mediated cell cycle control, with our team ready to assist you in integrating this reagent into your diagnostic or research workflows.