Anti-AKR7A3 Rabbit Polyclonal Antibody for WB, ELISA - O95154

Core Product Specifications and Parameters

Biological Background: AKR7A3 Function and Localization

• AKR7A3, also designated AFB1 aldehyde reductase 2 (AFB1-AR 2), is a member of the aldo-keto reductase family 7.
• It catalyzes the NADPH-dependent reduction of various carbonyl-containing compounds, including aldehydes and ketones.
• Specifically, it reduces the dialdehyde form of aflatoxin B1 (AFB1) into alcohol derivatives via monoaldehyde intermediates, thus preventing the formation of protein adducts that contribute to AFB1-induced toxicity.
• Subcellular localization is predominantly cytoplasmic.
• Tissue expression includes colon, kidney, liver, pancreas, adenocarcinoma, and endometrium.
• As an oxidoreductase, it plays a role in cellular detoxification processes.
• UniProt keywords highlight the presence of 3D structure data and phosphoprotein status, indicating post-translational modifications and structural elucidation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human AKR7A3 (amino acids 1-331), enabling detection of the endogenous protein.
• For western blotting, consider validating the antibody in tissues with known high expression such as liver or kidney, and use appropriate loading controls.
• When performing ELISA, optimize coating and detection conditions using purified recombinant protein or cell lysates.
• Due to rabbit polyclonal nature, expect reactivity with human, mouse, and rat samples as indicated by the cross-reactivity profile.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement platform for IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies and bulk ancillary reagents. This anti-AKR7A3 antibody supports research into detoxification pathways and toxicology, with our team ready to assist in sourcing rare targets and custom reagent needs. We streamline the path from concept to clinic for diagnostic manufacturers and research laboratories.