Anti-ATR Polyclonal Antibody for WB, IHC-P, ELISA - Q13535

Core Product Specifications and Parameters

Biological Background: ATR Function and Localization

• ATR is a serine/threonine protein kinase that recognizes the substrate consensus sequence [ST]-Q, activating checkpoint signaling upon DNA damage (PubMed:10597277, PubMed:10608806, PubMed:10859164).
  Related references: PMID:10597277, PMID:10608806, PMID:10859164

• ATR phosphorylates several downstream targets including BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1, and p53/TP53, thereby inhibiting DNA replication and mitosis while promoting DNA repair and apoptosis (PubMed:11114888, PubMed:11418864, PubMed:11865061).
  Related references: PMID:11114888, PMID:11418864, PMID:11865061

• It phosphorylates histone variant H2AX at Ser-139 at DNA damage sites, regulating the DNA damage response (PubMed:11673449).
  Related references: PMID:11673449

• Required for FANCD2 ubiquitination and critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed:15314022, PubMed:12526805).
  Related references: PMID:15314022, PMID:12526805

• Acts as a regulator of the S-G2 transition by restricting CDK1 activity during S-phase to prevent premature entry into G2 (PubMed:30139873).
  Related references: PMID:30139873

• Localizes to the nuclear envelope and acts as a mechanical stress sensor, mediating checkpoint signaling via CHEK1 phosphorylation; also promotes nuclear envelope rupture in response to DNA damage by phosphorylating LMNA at Ser-282 (PubMed:25083873, PubMed:37832547).
  Related references: PMID:25083873, PMID:37832547

• Involved in the inflammatory response to genome instability: localizes to micronuclei and phosphorylates LMNA at Ser-395, priming it for CDK1-mediated phosphorylation, leading to micronuclear envelope rupture and activation of the cGAS-STING pathway and type I interferon response (PubMed:37788673).
  Related references: PMID:37788673

• Ubiquitously expressed with highest levels in testis; isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400–500 of human ATR, a region involved in protein-protein interactions; the polyclonal serum may recognize multiple epitopes within this fragment.
• For Western blotting, expect a specific band at ~301 kDa; use a large molecular weight marker, low-percentage SDS-PAGE gel (6%), and extended wet transfer to improve resolution and transfer efficiency.
• Validate cross-reactivity with mouse and rat ATR by comparing lysates from positive controls; sequence identity is high but confirm with known cell lines or tissues.
• In immunohistochemistry (IHC-P), perform antigen retrieval (e.g., citrate buffer, pH 6.0) and include a no-primary or blocking peptide control to assess staining specificity.
• For ELISA, the antibody can be used as a detection reagent; pairing with a capture antibody against a distant epitope may enhance assay performance; validate with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for diagnostic manufacturers and research institutes, offering a comprehensive portfolio of IVD raw materials from validated antibody pairs to bulk reagents. This anti-ATR polyclonal antibody supports DNA damage and oncology research; we can assist with scaling up supply and customizing ancillary reagent kits to streamline your assay development workflow. Whether you need established antibody clones or rare-target sourcing, our integrated service bridges the gap from concept to clinic.