Anti-AKR1C4 Rabbit Polyclonal Antibody for WB, ELISA - P17516

Core Product Specifications and Parameters

Biological Background: AKR1C4 Function and Localization

• AKR1C4 (aldo-keto reductase family 1 member C4), also known as 3-alpha-hydroxysteroid 3-dehydrogenase type I (3-alpha-HSD1), chlordecone reductase (CDR), dihydrodiol dehydrogenase 4 (DD4), or HAKRA, is a cytosolic enzyme.
• It catalyzes the NADPH-dependent reduction of a broad range of ketosteroids with distinct positional and stereospecificity, producing 3alpha/beta-, 17beta-, and 20alpha-hydroxysteroids, and it inactivates 5alpha-dihydrotestosterone and progesterone by converting them to androstanediols and (20S)-hydroxypregn-4-en-3-one, respectively. Related references: PMID:10634139, PMID:10998348, PMID:11158055, PMID:14672942
• As part of the 'backdoor' androgen biosynthesis pathway, AKR1C4 cooperates with AKR1C2 to convert 5alpha-dihydroprogesterone to allopregnanolone, leading to 5alpha-dihydrotestosterone secretion essential for embryonic gonad differentiation into testis. Related references: PMID:21802064
• The enzyme regulates neurosteroid levels by reducing 5alpha-dihydroprogesterone and 5alpha-dihydrodeoxycorticosterone to allopregnanolone and THDOC, which modulate GABAA receptor activity and neural excitability. Related references: PMID:12604236
• It may contribute to adrenal androgen metabolism by reducing 11-keto-androgens (11K-Adione and 11KDHT) to their hydroxyl counterparts. Related references: PMID:31926269
• AKR1C4 can directly reduce sulfate- or glucuronide-conjugated androgens, participating in an alternative phase II metabolism pathway. Related references: PMID:19218247
• The enzyme catalyzes the detoxification of the pesticide chlordecone (kepone) to its alcohol, enhancing biliary excretion and reducing neurotoxicity. Related references: PMID:2427522
• AKR1C4 is expressed in the liver, fetal and adult testes, and adrenal glands, and localizes to the cytoplasm. Related references: PMID:10998348, PMID:11158055, PMID:7650035, PMID:21802064

Experimental Guidance and Technical Tips

• As the immunogen spans full-length AKR1C4, the polyclonal antibody may recognize multiple epitopes, facilitating detection of both native and denatured protein.
• For Western blot, a band near 37 kDa is expected under reducing conditions; verify in your sample system with appropriate controls.
• The antibody has been reported to react with human, mouse, and rat AKR1C4; cross-reactivity with other species should be validated in the relevant experimental context.
• In ELISA, consider testing the antibody as both capture and detection reagent, and optimize coating concentrations and blocking conditions for your specific assay.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research into steroid hormone pathways, neurosteroid biology, or androgen metabolism, CamelBio offers validated antibody pairs, optimized polyclonal antibodies like this anti-AKR1C4, and bulk ancillary reagents. Leverage our comprehensive supply capabilities to source rare-target raw materials and streamline your assay development.