Anti-AKR1C3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42330

Core Product Specifications and Parameters

Biological Background: AKR1C3 Function and Localization

• AKR1C3 (Aldo-keto reductase family 1 member C3), also designated 17-beta-HSD 5, DD3, PGFS, and HAKRb, is a cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids with broad substrate specificity. Related references: PMID:10998348, PMID:11165022, PMID:20036328
• Participates in classical and 'backdoor' androgen biosynthesis pathways: reduces Δ4-androstenedione to testosterone (classical) and androsterone to 5α-androstane-3α,17β-diol (backdoor), contributing to potent androgen production. Related references: PMID:10557352, PMID:10998348, PMID:11165022, PMID:20036328, PMID:9415401
• Reduces 5α-dihydrotestosterone (5α-DHT) to the weaker androgen 5α-androstane-3α,17β-diol, likely regulating ligand availability for androgen receptors. Related references: PMID:10557352, PMID:10998348, PMID:11165022
• Metabolizes 11-oxygenated androgens: reduces 11-keto-4-androstene-3,17-dione (11KA4) and 11-keto-5α-androstane-3,17-dione (11K-Adione) into potent androgens 11-ketotestosterone (11KT) and 11-ketodihydrotestosterone (11KDHT). Related references: PMID:31926269
• In estrogen metabolism, catalyzes the conversion of estrone to the potent estrogen 17β-estradiol. Related references: PMID:10998348, PMID:11165022, PMID:20036328
• Functions as a prostaglandin F2α synthase, with 11-ketoreductase and 9,11-endoperoxide reductase activities that reduce PGD2 and PGH2 to PGF2α. Related references: PMID:10622721, PMID:11165022, PMID:15047184
• Displays retinaldehyde reductase activity toward 9-cis-retinal. Related references: PMID:21851338
• Localizes to the cytoplasm and is expressed in many tissues, with highest levels in prostate and mammary gland; also found in adrenal gland, brain, kidney, liver, lung, and others.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human AKR1C3 protein (amino acids 1-323), suggesting potential recognition of multiple epitopes and robust detection.
• For Western blot (WB), use appropriate protein lysates from tissues with high AKR1C3 expression, such as prostate or mammary gland. Validate antibody performance in your specific sample system with positive and negative controls.
• The antibody has been reported to cross-react with mouse and rat AKR1C3; consider verifying reactivity in these species under your experimental conditions.
• Suitable for immunohistochemistry (IHC) on paraffin-embedded sections; antigen retrieval conditions may require optimization.
• For ELISA applications, ensure proper coating and detection conditions, and consider using recombinant AKR1C3 as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing manufacturers and researchers with validated antibodies, antigen pairs, and bulk ancillary reagents. This AKR1C3 polyclonal antibody supports steroid hormone metabolism and oncology research, and we can assist with sourcing complementary reagents such as matched antigen controls, enzyme conjugates, and rare target raw materials. Let CamelBio streamline your development pipeline from concept to clinic.