Anti-ACVR1C Polyclonal Antibody for WB, IHC-P, ELISA - Q8NER5

Core Product Specifications and Parameters

Biological Background: ACVR1C Function and Localization

• ACVR1C (Activin receptor type-1C), also called ALK7 or ACTR-IC, is a serine/threonine protein kinase that operates as a type I receptor in the TGF-beta superfamily signaling cascade.
• The receptor associates with type II receptors to form a heterotetrameric complex; upon ligand binding, type II receptors phosphorylate and activate ACVR1C, leading to autophosphorylation and subsequent recruitment and phosphorylation of SMAD2 and SMAD3 transcription factors.
• ACVR1C serves as a receptor for activin AB, activin B, activin E, and the morphogen NODAL; NODAL binding specifically triggers pro-apoptotic and anti-proliferative signaling through AKT pathway suppression and Smad2 activation in various cell types including pancreatic beta-cells and neurons. Related references: PMID:15531507 PMID:15150278
• Beyond its role in cell growth regulation, ACVR1C positively regulates macrophage activation by down-regulating PPARG expression (By similarity).
• The protein is a single-pass transmembrane receptor localized at the cell membrane and contains an intracellular kinase domain that binds ATP and requires divalent cations such as magnesium or manganese for catalytic activity.
• ACVR1C is expressed in multiple tissues, notably pancreas, heart, colon, small intestine, ovary, and brain regions (hippocampus, medulla oblongata, putamen); all four alternative splice isoforms (1-4) are detected in the placenta throughout pregnancy.
• Post-translational modifications include phosphorylation at multiple residues; the kinase activity and downstream signaling are tightly regulated by phosphorylation events.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 22-113), suggesting the antibody is well-suited for detecting full-length ACVR1C on cell surfaces and in lysates; it may also recognize soluble receptor fragments.
• For Western blot, a band at ~55 kDa (predicted molecular weight) is expected; consider optimizing blocking and antibody dilution using positive controls like pancreatic or placental tissue lysates.
• In immunohistochemistry (IHC-P), paraffin-embedded sections may require heat-induced antigen retrieval; strong staining is likely in pancreatic islets, placental trophoblasts, or neuronal tissues based on reported expression patterns.
• ELISA applications can be designed using the antibody as a capture or detection reagent; pairing with a monoclonal antibody against a distinct epitope may improve assay specificity.
• Due to reported cross-reactivity with mouse and rat, this antibody can be used in rodent models; however, sequence alignment of the immunogen region across species is recommended to confirm epitope conservation.
• Always validate antibody performance in your specific sample type and assay conditions; include appropriate controls (e.g., knockdown or knockout cell lines) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials and support services, helping diagnostic manufacturers, labs, and researchers move from concept to clinic efficiently. For ACVR1C, a key receptor in TGF-beta signaling and apoptosis, CamelBio can supply validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents to accelerate your assay development. Our sourcing expertise also covers rare targets, ensuring you have a reliable partner for high-quality reagents at every stage.