Anti-AK3 Rabbit Polyclonal Antibody for WB, ELISA - Q9UIJ7

Core Product Specifications and Parameters

Biological Background: AK3 Function and Localization

• AK3, also known as adenylate kinase 3 or AK3L1, is a mitochondrial enzyme belonging to the adenylate kinase family.
• It catalyzes the reversible transfer of a phosphate group from GTP to AMP, producing GDP and ADP, thereby playing a key role in recycling GTP in the mitochondrial matrix. Related references: PMID:11485571 PMID:32822537
• In addition to GTP, AK3 can utilize ITP as a phosphate donor. Related references: PMID:11485571
• This activity is critical for recycling GTP to GDP, which is necessary for the TCA cycle in the mitochondrial matrix (Probable).
• The enzyme is localized in the mitochondrial matrix, consistent with its role in nucleotide metabolism within the organelle.
• AK3 is highly expressed in heart, skeletal muscle, and liver, with moderate expression in pancreas and kidney, and low expression in placenta, brain, and lung.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data.
• The protein may undergo alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, consider using mitochondrial extracts or lysates from heart or skeletal muscle tissues as positive controls, as AK3 is highly expressed in these tissues.
• A specific band around 26 kDa may be observed; validate the antibody in your sample system to confirm specificity.
• The antibody cross-reacts with mouse and rat, enabling its use in samples from these species; however, appropriate controls are recommended.
• For ELISA, the antibody can be used for detection of AK3 in biological samples; optimization of coating and detection conditions may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. The AK3 antibody supports research in mitochondrial metabolism and nucleotide signaling, areas critical for understanding energy-related disorders. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and production workflows.