Anti-CA3 Polyclonal Antibody for WB, IF/ICC, ELISA - P07451

Core Product Specifications and Parameters

Biological Background: CA3 Function and Localization

• Encodes human carbonic anhydrase 3 (CA3/CAIII), also known as carbonate dehydratase III, a zinc metalloenzyme catalyzing the reversible hydration of carbon dioxide (CO₂ + H₂O ⇌ HCO₃⁻ + H⁺).
• CA3 is a muscle-specific cytoplasmic protein, predominantly expressed in skeletal muscle, consistent with its role in buffering pH changes during contraction.
• Belongs to the alpha-carbonic anhydrase family, a group of enzymes that maintain acid-base balance and facilitate CO₂ transport; unlike CA1 and CA2, CA3 exhibits lower catalytic activity and possible dual functions.
• Subcellular localization is primarily cytoplasmic, as indicated by UniProt annotation and lack of transmembrane domains.
• Post-translational modifications include acetylation, glutathionylation, and phosphorylation, potentially regulating enzyme activity or stability.
• Molecular weight of the unmodified monomer is ~29.6 kDa; the observed ~30 kDa band in SDS-PAGE likely reflects the mature protein.

Experimental Guidance and Technical Tips

• The immunogen used is a full-length recombinant human CA3 protein (amino acids 1-260), which may yield a polyclonal antibody recognizing multiple epitopes; consider this when analyzing band patterns in western blot.
• This antibody is suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA applications as reported; validate cross-reactivity in human, mouse, and rat tissue samples relevant to your experimental system.
• For optimal results, titrate the antibody using a serial dilution (e.g., 1:500–1:2000 for WB) and include appropriate positive and negative controls, especially when working with muscle-specific samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering seamless access to anti-CA3 antibodies for research in muscle physiology and metabolic pH regulation. Our supply capabilities span validated antibody pairs, bulk ancillary reagents, and rare target raw materials, empowering diagnostic manufacturers from concept to clinic. Whether you require routine carbonic anhydrase reagents or specialized sourcing, CamelBio bridges the gap with reliable procurement solutions.