Anti-XK Polyclonal Antibody for WB, IF/ICC, ELISA - P51811

Core Product Specifications and Parameters

Biological Background: XK Function and Localization

• The XK gene encodes a membrane transport protein, also known as XKR1 or Kx antigen, which functions as an adapter at the endoplasmic reticulum (ER) and is a component of the Kell blood group complex.
• XK recruits the lipid transfer protein VPS13A from lipid droplets to the ER membrane, facilitating lipid transport at membrane contact sites.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its role in mediating ER–lipid droplet contacts.
• High expression is observed in skeletal muscle, heart, brain, and pancreas, while lower levels are found in placenta, lung, liver, and kidney.
• XK variants are associated with McLeod syndrome, a neuroacanthocytosis disorder, highlighting its clinical relevance.
• Characterized by transmembrane helices, disulfide bonds, and phosphorylation sites, XK is classified as an amino acid transporter and a blood group antigen (Kx).

Experimental Guidance and Technical Tips

• For Western blot, use a positive control such as skeletal muscle or brain lysate to verify detection of the ~51 kDa band; optimize blocking and antibody dilution empirically.
• In immunofluorescence/ICC, membrane permeabilization and appropriate fixation are essential; co-staining with an ER marker can confirm expected subcellular localization.
• The immunogen sequence (aa 59–138) suggests suitability for detecting native protein in ELISA; include recombinant protein and negative controls to assess specificity.
• Validate antibody performance in your target species (human, mouse, rat) and consider antigen retrieval methods for immunohistochemical applications where applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined, single-point access to IVD raw materials, including validated polyclonal antibodies like this anti-XK antibody, optimized matched pairs, and bulk ancillary reagents. Whether your work focuses on ER membrane adapter proteins, blood group antigens, or lipid transport mechanisms, our sourcing network and technical support help bridge the gap from early-stage concept to clinical application.