Anti-AKT3 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y243

Core Product Specifications and Parameters

Biological Background: AKT3 Function and Localization

• AKT3 encodes RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3 or PKB gamma, a member of the AKT kinase family alongside AKT1 and AKT2.
• As a serine/threonine kinase, AKT3 regulates diverse cellular processes including metabolism, proliferation, cell survival, growth, and angiogenesis through phosphorylation of numerous downstream substrates.
• AKT3 is particularly important for brain development and is critical for the viability of malignant glioma cells, highlighting its role in neurobiology and oncology.
• The kinase is involved in the regulation of MMP13 expression via IL13 and coordinates mitochondrial biogenesis with growth factor-induced energy demands.
• Down-regulation of AKT3 leads to decreased phosphorylation of BAD, thereby promoting caspase-dependent apoptosis.
• Subcellularly, AKT3 localizes to the nucleus, cytoplasm, and membrane, reflecting its multifaceted signaling roles.
• In adult tissues, AKT3 is highly expressed in brain, lung, and kidney, with lower levels in heart, testis, and liver; fetal expression is high in heart, liver, and brain, but absent in kidney.
• Post-translational modifications and regulatory features include phosphorylation, acetylation, ubiquitination, and disulfide bond formation, consistent with its tight control in signaling cascades.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 90–190) within the N-terminal portion of human AKT3, suggesting the antibody may recognize an epitope that is conserved across human, mouse, and rat as indicated by the cross-reactivity data.
• For Western blot analysis, consider using standard lysate preparation and loading controls, and validate the antibody in your specific cell or tissue model. Given the predicted molecular weight of 56 kDa, ensure gel resolution is optimized for this range.
• In ELISA applications, test the antibody in both direct and indirect formats; titration is recommended to determine the optimal working concentration for your assay conditions.
• Because AKT3 expression varies across tissues and developmental stages, include appropriate positive and negative controls (e.g., brain vs. liver lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD manufacturers and research laboratories, offering streamlined access to a wide array of raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This AKT3 polyclonal antibody supports investigations into AKT signaling, cancer biology, and neuroscience, and can be procured alongside complementary products such as AKT1/2 antibodies, phospho-specific detection reagents, and ELISA development kits. Our integrated supply chain and technical support simplify the sourcing process, helping you advance from concept to clinical application efficiently.