Anti-AKR1C3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P42330

Core Product Specifications and Parameters

Biological Background: AKR1C3 Function and Localization

• Aldo-keto reductase family 1 member C3 (AKR1C3) catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids with broad substrate specificity, primarily generating 17beta-hydroxysteroids. Related references: PMID:10998348, PMID:11165022, PMID:20036328
• In androgen metabolism, AKR1C3 converts delta4-androstenedione to testosterone in the classical pathway and reduces androsterone to 5alpha-androstane-3alpha,17beta-diol in the backdoor pathway, contributing to potent androgen biosynthesis. Related references: PMID:10557352, PMID:10998348, PMID:9415401
• It regulates androgen signaling by reducing 5alpha-DHT to 5alpha-androstane-3alpha,17beta-diol, and catalyzes the conversion of estrone to 17beta-estradiol in estrogen metabolism. Related references: PMID:10557352, PMID:10998348, PMID:11165022
• AKR1C3 functions as a prostaglandin F2alpha synthase, reducing PGD2 to 11beta-PGF2alpha and PGH2 to PGF2alpha. Related references: PMID:10622721, PMID:11165022, PMID:15047184
• It exhibits retinaldehyde reductase activity toward 9-cis-retinal and can reduce various carbonyl compounds in vitro. Related references: PMID:21851338
• AKR1C3 is localized to the cytoplasm and is highly expressed in prostate, mammary gland, and other tissues, suggesting roles in tissue-specific hormone metabolism and related diseases.
• Alternative splicing of the AKR1C3 gene generates multiple protein isoforms, expanding its functional diversity.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 37 kDa. Use lysates from human or mouse tissues with high AKR1C3 expression (e.g., prostate, mammary gland) as positive controls.
• In immunofluorescence (IF/ICC), AKR1C3 shows cytoplasmic staining. Optimize fixation and permeabilization conditions, and validate specificity with appropriate negative controls (e.g., knockout cell lines).
• For ELISA, this monoclonal antibody can be used as a capture or detection reagent. Titrate antibody concentrations and determine optimal coating and blocking conditions for your assay.
• The immunogen peptide spans amino acids 200–300, a region with potential epitope specificity. Cross-reactivity with related aldo-keto reductases should be assessed in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw material sourcing, supporting diagnostic manufacturers and researchers from concept to commercialization. For AKR1C3-related projects, we supply this validated monoclonal antibody along with compatible antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. Our team ensures reliable supply and technical consultation, making therapeutic monitoring and hormonal disorder research more accessible.