YKT6 Rabbit Polyclonal Antibody for WB and ELISA - O15498

Core Product Specifications and Parameters

Biological Background: YKT6 Function and Localization

• YKT6 is a synaptobrevin homolog that functions as a vesicular soluble NSF attachment protein receptor (v-SNARE), mediating vesicle docking and fusion to specific target membranes. Related references: PMID:9211930 PMID:15215310
• It operates in two major trafficking pathways: endoplasmic reticulum (ER) to Golgi transport (as part of a SNARE complex with GOSR1, GOSR2, and STX5) and early/recycling endosome to trans-Golgi network (TGN) transport (with BET1L, GOSR1, and STX5). Related references: PMID:15215310
• YKT6 possesses intrinsic S-palmitoyl transferase activity, enabling its own palmitoylation and contributing to membrane association. Related references: PMID:15479160 PMID:15044687
• The protein is essential for the structural and functional integrity of the Golgi apparatus. Related references: PMID:32128853
• Subcellularly, YKT6 localizes to the cytoplasm, specifically the cytosol, and to membranes of cytoplasmic vesicles and the Golgi apparatus.
• The protein undergoes multiple post-translational modifications, including palmitoylation, prenylation, and methylation, which regulate its membrane targeting and function.
• Alternative splicing generates multiple isoforms, and the protein contains a coiled-coil domain typical of SNARE proteins.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 100–198) of human YKT6, which avoids transmembrane domains and may yield high specificity; cross-reactivity with mouse and rat has been observed, suggesting conserved epitopes.
• For Western blot, a band at approximately 22 kDa is expected; however, post-translational modifications (e.g., palmitoylation, prenylation) may cause slight mobility shifts—validate using appropriate controls and reducing conditions.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent; consider pairing with a compatible monoclonal antibody to develop a sandwich assay if desired.
• When using cell or tissue lysates, inclusion of fresh protease inhibitors and mild detergents may help preserve the native conformation and epitope accessibility.
• This antibody has not been tested in other applications such as immunoprecipitation or immunofluorescence; pilot experiments are recommended before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by offering validated polyclonal antibodies like this anti-YKT6 antibody, along with complementary reagents such as recombinant proteins, ELISA kits, and matched antibody pairs for vesicle trafficking research. Our comprehensive portfolio covers bulk ancillary materials and rare-target sourcing, enabling diagnostic developers and research laboratories to accelerate assay development from concept to clinic. Partner with us to secure consistent, high-quality supplies tailored to your SNARE protein or Golgi biology projects.