Anti-IL-6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05231

Core Product Specifications and Parameters

Biological Background: IL-6 Function and Localization

• IL-6 (Interleukin-6) is a pleiotropic cytokine with alternative names including B-cell stimulatory factor 2 (BSF-2), CTL differentiation factor (CDF), Hybridoma growth factor, and Interferon beta-2 (IFN-beta-2).
• It plays a central role in the acute phase response, rapidly produced by myeloid cells upon pathogen recognition, contributing to host defense but also to disease pathology when excessively synthesized.
• IL-6 is critical for adaptive immunity, driving B cell differentiation into immunoglobulin-secreting cells and regulating CD4+ T cell subsets, including the differentiation of T follicular helper (Tfh) and Th17 cells.
• It acts as a key regulator of bone homeostasis and angiogenesis by inducing VEGF, enhancing vascular permeability (Probable). Related references: PMID:12794819 PMID:17075861
• IL-6 functions as a myokine, released into the bloodstream after muscle contraction to increase lipolysis and improve insulin resistance (Probable). Related references: PMID:20823453
• It regulates iron metabolism by controlling HAMP/hepcidin expression during inflammation or bacterial infection. Related references: PMID:15124018
• The protein is secreted and undergoes post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation (Keywords: Secreted, Glycoprotein, Disulfide bond, Phosphoprotein).
• Tissue expression includes production by skeletal muscle (Tissue specificity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 30-212 of the mature IL-6 protein; this region is expected to recognize the secreted, biologically active form of IL-6.
• For Western blotting (WB), consider using denaturing and reducing conditions and validate with positive controls such as stimulated macrophage lysates or recombinant IL-6.
• In immunofluorescence (IF/ICC), due to the secreted nature of IL-6, treatment with a secretion inhibitor (e.g., brefeldin A) before fixation may enhance detection; co-staining with organelle markers can provide spatial context.
• For ELISA applications, verify the antibody’s suitability as a capture or detection reagent using appropriate recombinant protein standards and matched antibody pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This IL-6 rabbit polyclonal antibody supports research and assay development in immunology and inflammation. For your diagnostic or laboratory needs, CamelBio provides a streamlined bridge from concept to clinical application.