Anti-PARD6A Rabbit Polyclonal Antibody for WB and ELISA - Q9NPB6

Core Product Specifications and Parameters

Biological Background: PARD6A Function and Localization

• PARD6A (Partitioning defective 6 homolog alpha), also known as PAR6C or TIP-40, is an adapter protein that plays critical roles in asymmetrical cell division and cell polarization.
• It participates in epithelial tight junction assembly by associating with PARD3, which may prevent PARD3 interaction with F11R/JAM1, thereby regulating tight junction formation.
• The PARD6-PARD3 complex serves as a link between GTP-bound Rho small GTPases and atypical protein kinase C (aPKC) proteins. Related references: PMID:10873802
• PARD6A regulates centrosome organization and is essential for recruiting key proteins that control centrosomal microtubule organization. Related references: PMID:20719959
• Subcellularly, PARD6A localizes to the cytoplasm, cell membrane, ruffles, tight junctions, and the centrosome/centriolar satellites.
• It is highly expressed in pancreas, skeletal muscle, brain, and heart, with weaker expression in kidney and placenta.
• Post-translational modifications include phosphorylation and possible ubiquitination (Ubl conjugation), and multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human PARD6A. This design targets a specific segment that may recognize multiple isoforms, but users should verify reactivity in their specific experimental context.
• For Western blotting, the predicted molecular weight is approximately 37 kDa; consider possible phosphorylation or cleavage that may affect migration.
• This antibody is reported to cross-react with mouse PARD6A, making it suitable for mouse model studies. However, always validate species-specific reactivity with appropriate positive and negative controls.
• In ELISA applications, consider coating the target peptide or recombinant protein to assess binding affinity and specificity. Titration of both coating antigen and antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers and research institutions by streamlining the procurement of critical raw materials like anti-PARD6A antibodies, which support deep investigations into cell polarity, tight junction dynamics, and centrosome biology. Our one-stop platform offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target biologicals, all coupled with technical consultation to accelerate your assay development and diagnostic pipeline.