Anti-ITGB6 Rabbit Polyclonal Antibody for WB, ELISA - P18564

Core Product Specifications and Parameters

Biological Background: Integrin beta-6 Function and Localization

• ITGB6 encodes integrin beta-6, which associates with alpha-V to form the heterodimeric receptor integrin alpha-V:beta-6 (ITGAV:ITGB6).
• ITGAV:ITGB6 serves as a receptor for fibronectin and cytotactin, recognizing the R-G-D motif. Its clathrin-mediated internalization promotes carcinoma cell invasion. Related references: PMID:17158881, PMID:17545607
• This integrin mediates R-G-D-dependent release and activation of latent TGF-beta-1 from LAP, a critical step in TGF-beta signaling. Related references: PMID:15184403, PMID:22278742, PMID:28117447
• ITGAV:ITGB6 also acts as a receptor for fibrillin-1 (FBN1) and supports R-G-D-dependent cell adhesion to FBN1. Related references: PMID:17158881
• In microbial infection, ITGAV:ITGB6 acts as a host receptor for Coxsackievirus A9, Coxsackievirus B1, and Herpes simplex virus-1 (HHV-1). Related references: PMID:24367260
• Subcellularly, the protein localizes to the cell membrane and focal adhesions, functioning as a transmembrane adhesion receptor.
• Key structural features include EGF-like domains, disulfide bonds, and N-glycosylation sites; it requires divalent cations (Ca²⁺, Mg²⁺) for activity.
• Alternative splicing yields multiple isoforms, and mutations in ITGB6 are associated with amelogenesis imperfecta.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic tail (aa 732–788) of human ITGB6; as a rabbit polyclonal, epitope recognition may vary across isoforms and species, so validation in the target sample is recommended.
• For Western blot, a band of approximately 86 kDa is expected; due to glycosylation, the observed molecular weight may be slightly higher. Use reducing conditions and appropriate membrane protein extraction protocols.
• For ELISA, recombinant ITGB6 protein or cell lysates from ITGB6-expressing lines can be used as coating antigens; cross-reactivity with human, mouse, and rat allows multi-species assay development.
• The antibody is supplied in liquid form, shipped on ice bags; upon receipt, store at -20°C and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop platform providing diagnostic manufacturers and research labs with high-quality IVD raw materials, technical services, and consulting from concept to clinic. This anti-ITGB6 polyclonal antibody supports cell adhesion, cancer invasion, TGF-beta biology, and viral entry studies. Beyond single antibody supply, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development and manufacturing pipelines.