p70 S6 Kinase 1 Rabbit Polyclonal Antibody for WB - P23443

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 1 Function and Localization

• RPS6KB1, also known as p70S6K1, is a serine/threonine-protein kinase encoded by the RPS6KB1 gene.
• Functions as a critical downstream effector of mTORC1 signaling, promoting cell proliferation, growth, and cell cycle progression by phosphorylating translation regulators including EIF4B, RPS6, and EEF2K. Related references: PMID:11500364, PMID:12801526, PMID:14673156
• Regulates translation initiation by dissociating from the EIF3 complex upon mTORC1-mediated phosphorylation and activating components of the pre-initiation complex; also promotes degradation of the translational repressor PDCD4. Related references: PMID:16286006, PMID:17053147
• Phosphorylates and inhibits pro-apoptotic BAD to promote cell survival, and modulates its own activity via URI1 phosphorylation and phosphatase recruitment. Related references: PMID:17936702
• Participates in negative feedback loops by phosphorylating mTORC2 components (SIN1, MTOR, RICTOR) and DEPTOR, thus dampening AKT signaling. Related references: PMID:15899889, PMID:19720745, PMID:19935711, PMID:22017876
• Displays diverse subcellular localization including cytoplasm, nucleus, mitochondrial outer membrane, and synaptic compartments, facilitating its multiple regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 426–525 of human P70 S6K, a region that may be critical for antibody specificity; consider verifying specificity in your experimental system.
• Applications include western blotting (WB; predicted MW ~59 kDa), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA; optimal working dilutions should be determined empirically.
• Cross-reactivity with mouse and rat orthologs is indicated, supporting translational research across these species; validate performance in the relevant biological context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier supplier of IVD raw materials, providing one-stop access to high-quality antibodies and ancillary reagents for diagnostic development. This anti-p70 S6 Kinase 1 antibody supports studies in mTOR signaling, translation regulation, and cell growth. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and rare-target sourcing to accelerate your project from concept to clinic.