Anti-Cytokeratin 6 (KRT6) Rabbit Polyclonal Antibody for WB and ELISA - P02538

Core Product Specifications and Parameters

Biological Background: Cytokeratin 6 (KRT6) Function and Localization

• KRT6A (Cytokeratin 6A) is a type II keratin that forms obligatory heteropolymers with type I keratins KRT16 and KRT17, assembling into intermediate filament networks critical for maintaining the structural integrity of stratified epithelia, particularly in the epidermis, nail bed, and oral mucosa. Related references: PMID:11886499, PMID:17719747, PMID:7545493
• During wound healing, KRT6A is rapidly induced in keratinocytes at the wound edge and participates in cytoskeletal reorganization during re-epithelialization, facilitating the restoration of the epithelial barrier.
• KRT6A negatively regulates collective keratinocyte migration by stabilizing non-muscle myosin MYH9 and desmoplakin, altering cell adhesion dynamics, and by inhibiting SRC activity, which reduces the speed and directionality of epithelial sheet movement.
• Subcellularly, KRT6A is localized to the cytoplasm, consistent with its role as an intermediate filament component.
• At the protein level, KRT6A is expressed in the corneal epithelium, indicating tissue-specific functional requirements.
• Pathogenic variants in KRT6A are associated with ectodermal dysplasia and palmoplantar keratoderma, as KRT6A is essential for the integrity of palmoplantar, nail unit, and oral mucosa. Related references: PMID:11886499, PMID:17719747, PMID:7545493

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-100) of human KRT6A, which may confer isoform-specific recognition; however, due to high sequence homology among type II keratins, cross-reactivity with KRT6B, KRT6C, or other keratins should be evaluated in the relevant sample system.
• For Western blot, a band around 60 kDa is expected; consider using human epithelial cell lines (e.g., HaCaT, A431) or skin/corneal tissue lysates as positive controls.
• This polyclonal antibody is suitable for ELISA; for sandwich ELISA development, pairing with a monoclonal detection antibody is recommended, and optimal dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. With this anti-Cytokeratin 6 antibody, we support your research into epithelial biology and keratin-related disorders. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development.