Anti-SYT6 Rabbit Polyclonal Antibody for WB, ELISA - Q5T7P8

Core Product Specifications and Parameters

Biological Background: Synaptotagmin-6 Function and Localization

• Synaptotagmin-6 (SYT6/SytVI) is a member of the synaptotagmin family of calcium-sensing proteins, which contain tandem C2 domains for Ca²⁺ and phospholipid binding.
• SYT6 may function in Ca²⁺-dependent exocytosis, regulating the fusion of secretory vesicles with the plasma membrane during neurotransmission and hormone secretion.
• The protein is localized to cytoplasmic vesicles, secretory vesicles, and synaptic vesicle membranes, as well as the cytosol and cell membrane, consistent with a role in vesicular trafficking.
• SYT6 has been implicated in the acrosomal reaction of sperm, where it may mediate calcium-triggered exocytosis (By similarity).
• It is a phosphoprotein with a transmembrane helix, metal-binding capacity (Ca²⁺), and disulfide bonds; it also undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-120) unique to SYT6; consider validating specificity against other synaptotagmin isoforms to confirm selectivity.
• For WB, expect a band near 57 kDa; use appropriate loading controls and test in relevant human, mouse, or rat tissue lysates to verify endogenous expression.
• ELISA performance can vary with coating conditions; titrate the antibody and optimize blocking steps to minimize polyclonal background.
• Although cross-reactivity with mouse and rat is reported, always verify using positive controls from each species to ensure consistent recognition in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, including this anti-SYT6 antibody for neuroscience and exocytosis research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to support diagnostic development and research applications.